Boschniakia rossica boschnaloside inhibition of epithelial-mesenchymal transition of hepatoma SMMC-7721 cells
10.3969/j.issn.1671-7856.2024.11.002
- VernacularTitle:草苁蓉纳拉苷对SMMC-7721肝癌细胞上皮-间质转化的抑制作用
- Author:
Aihua JIN
1
;
Jiebo ZHU
;
Jishu QUAN
Author Information
1. 延边大学附属医院(延边医院),吉林 延吉 133000
- Publication Type:Journal Article
- Keywords:
Boschniakia rossica;
Boschniakia rossica boschnaloside;
SMMC-7721 cells;
EMT;
STAT3
- From:
Chinese Journal of Comparative Medicine
2024;34(11):11-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of Boschniakia rossica boschnaloside(BRBN)on the epithelial-mesenchymal transition(EMT),invasion,and migration of human hepatoma SMMC-7721 cells.Methods Transforming growth factor β1(TGF-β1)was used to induce an EMT model of SMMC-7721 cells.The cells were divided into a control group,model group,and BRBN group.Cell migration was detected with a wound healing test,and cell invasion was observed by Transwell chamber assay.The expression of E-cadherin,N-cadherin,Vimentin,Slug,Twist1,ZEB1,and signal transducers and activators of transcription 3(STAT3)were determined with the Western blot method.Results TGF-β1 resulted in spindle-shaped SMMC-7721 cells,down-regulated the expression of E-cadherin,and up-regulated the expression of N-cadherin and vimentin,suggesting that SMMC-7721 cells obtained a mesenchymal phenotype.Compared with the model group,the BRBN group SMMC-7721 cells'expression of E-cadherin was significantly increased,their expression of N-cadherin and vimentin were reduced,and their expression of Slug,Twist1,and ZEB1,as well as STAT3 phosphorylation,were down-regulated.Conclusions BRBN inhibits the invasion and migration of human hepatoma SMMC-7721 cells,possibly by reversing EMT through the STAT3 pathway.
