Research on the inhibitory effect and related mechanisms of Peripla-neta americana extract on prostate cancer cells
10.12092/j.issn.1009-2501.2024.11.007
- VernacularTitle:美洲大蠊提取物对前列腺癌细胞的抑制作用及相关机制研究
- Author:
Saizhu DUAN
1
;
Feng XU
;
Haiyan CHEN
;
Jiamao CHENG
Author Information
1. 大理大学临床医学院,大理 671003,云南
- Publication Type:Journal Article
- Keywords:
Periplaneta americana extracts;
pros-tate cancer;
zinc-alpha2-glycoprotein;
cell prolifera-tion;
cell migration
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(11):1260-1266
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the mechanism of Periplaneta americana extracts(PA-CⅡ-3)on anti-prostate cancer.METHODS:Prostate cancer cells PC3 in logarithmic growth phase were divided into a control group and an experimental group.The PA-CⅡ-3 solutions of 1 000,1 200,1 400,1 600,1 800,and 2 000 μg/mL were added successively to the PC3 cells in experimental group,for screening out the appropriate drug concentration and best time to intervene by CCK-8 assay.And then,PA-CⅡ-3 so-lution at 1 400 μg/mL was continuously intervened in PC3 cells for 72 hours.The cell proliferation abili-ty was detected by CCK-8 assay and plate colony formation assay,the cell migration ability was test-ed by scratch wound assay,and the mRNA and pro-tein expressions of ZAG were detected by Real time qPCR and Western blot methods,respectively.RE-SULTS:After PA-CⅡ-3 intervention,the proliferation and migration abilities of PC3 cells were significant-ly inhibited,and the mRNA and protein expression levels of ZAG in PC3 cells were promoted.CONCLU-SION:PA-CⅡ-3 can play the role of anti-prostate cancer by inhibiting the proliferation and migration of PC3 cells,and upregulating ZAG.
