Resveratrol activates autophagy and inhibits pyroptosis to alleviate cerebral ischemia-reperfusion injury
10.12092/j.issn.1009-2501.2024.11.005
- VernacularTitle:白藜芦醇激活自噬抑制焦亡减轻脑缺血再灌注损伤
- Author:
Xiaoliang ZHANG
1
;
Saihong GAO
;
Yingchun YANG
;
Qingyu WANG
;
Shuyu JIA
Author Information
1. 山西医科大学汾阳学院解剖教研室,汾阳 032200,山西
- Publication Type:Journal Article
- Keywords:
resveratrol;
ischemia-reperfusion in-jury;
autophagy;
pyroptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2024;29(11):1240-1248
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the potential of resveratrol(Res)to alleviate cerebral ischemia-re-perfusion injury(CIRI)by activating autophagy.METHODS:One hundred SD rats were purchased and subjected to middle cerebral artery occlusion/reperfusion(MCAO/R)using the suture method.Rats with failed surgeries were excluded,and they were randomly divided into seven groups:Sham(S)group,Model(M)group,Resveratrol pretreatment(Res)group,3-methyladenine(3-MA)group,Resve-ratrol+3-methyladenine(Res+3-MA)group,Z-YVAD group,and Res+Z-YVAD group,with 12 rats in each group.Neurological deficits and brain dam-age were assessed using Longa scoring,TTC stain-ing,and measurement of infarct volume.Immuno-histochemistry,immunofluorescence,and immu-noblotting were employed to detect the expression of autophagy-related proteins Beclin-1,LC3-Ⅱ,P62,and pyroptosis-related proteins NLRP3,caspase-1,GSDMD,IL-1β.RESULTS:Resveratrol pretreatment improved neurological deficits and reduced infarct volume in rats.It upregulated autophagy-related proteins Beclin-1,LC3-Ⅱ,downregulated P62,and pyroptosis-related proteins NLRP3,caspase-1,GSD-MD,IL-1β expression.The autophagy inhibitor 3-MA completely reversed the above effects of Res.After adding the pyroptosis inhibitor Z-YVAD,the changes in autophagy-related proteins Beclin-1,LC3-Ⅱ,and P62 were not significant,while the ex-pression of NLRP3,caspase-1,GSDMD,IL-1β signifi-cantly decreased.Neurological deficits worsened,and infarct volume increased in rats.CONCLUSION:Res attenuates cerebral ischemia-reperfusion injury by activating autophagy and inhibiting pyroptosis,possibly through the regulation of the P62.
