Mechanism of resistance exercise in improving sarcopenia in SAMP8 mice through the JAK2/STAT5a pathway
10.3969/j.issn.1001-1242.2024.12.003
- VernacularTitle:抗阻运动经JAK2/STAT5a改善SAMP8小鼠肌少症的作用机制
- Author:
Bo QU
1
;
Lunyu LI
;
Ying HUANG
Author Information
1. 成都体育学院运动医学与健康学院,四川省成都市,610041
- Publication Type:Journal Article
- Keywords:
resistance exercise;
sarcopenia;
JAK/STATa;
transcriptome;
IL-6;
SAMP8 mice
- From:
Chinese Journal of Rehabilitation Medicine
2024;39(12):1756-1765
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of resistance exercise improving sarcopenia through JAK2/STAT5a path-way based on transcriptomics.Method:Sixteen 28-week-old male SAMP8 mice were randomly divided into an aging model group(Group M)and a resistance training group(Group R);another eight age-matched male anti-aging mice(SAMR l)were set up as Group C(the young control group).Groups C and Group M were routinely housed without any interventions,and Group R underwent 8 weeks of incremental load ladder climbing training.The relative grip strength and rotating rod time were measured every two weeks,and at the end of the intervention,the relative muscle mass of the mice was tested and the cross-sectional area of muscle fibers was observed;RNA-seq technology was used to build libraries for transcriptome sequencing,to screen for differentially expressed genes,and to enrich relevant physiological characteristics and highly relevant signaling pathways through GO,KEGG enrichment,with PPI screening for related core genes;RT-qPCR was used to detect mRNA expression of IL-6,Jak2,Stat5a and Socs3 in skeletal muscle.Result:①Relative grip strength,rotating rod time and muscle mass of the mice showed that group M was significantly lower than group C(P＜0.001),while group R was significantly higher than group M(P＜0.05);The HE staining of muscle cross-sectional area showed group M was significantly lower than group C(P＜0.001),while group R was significantly higher than group M(P＜0.05);Transcriptomic differential gene screening:a total of 572 DEGs were screened between groups R and M,with 231 mRNA expressions upregu-lated and 341 mRNA expressions downregulated in group R compared to group M(FC≥0,P＜0.05);JAK-STAT signaling pathway was found to be highly expressed by enrichment analysis of DEGs GO and KEGG;PPI screened for the core genes of sarcopenia core genes JAK2,STAT5a.RT-qPCR results showed that com-pared to group C,the gene expression levels of IL-6,Jak2,and STAT5a in skeletal muscle in group M were significantly increased(P＜0.01),and the expression level of Socs3 was significantly decreased(P＜0.01);com-pared with group M,the levels of IL-6 mRNA,JAK2,and STAT5a in skeletal muscle in group R were sig-nificantly decreased(P＜0.05),while the levels of Socs3 were significantly elevated(P＜0.01).Conclusion:Resistance exercise can improve sarcopenia in SAMP8 mice by inhibiting the JAK2/STAT5a pathway.
