Study on the effect and mechanism of Biejiajian pill on the malignant biological behaviors of hepatocellular carcinoma Huh7 cells
10.3969/j.issn.1671-7856.2025.07.005
- VernacularTitle:鳖甲煎丸对肝癌Huh7细胞恶性生物学行为的影响及机制研究
- Author:
Yiheng LI
1
;
Junjie XU
;
Tao LAN
;
Xin LI
;
Ronghua ZHANG
;
Yanan XIONG
;
Lihua ZHU
;
Guangling ZHANG
Author Information
1. 华北理工大学基础医学院,河北省慢性疾病重点实验室,河北唐山 063210
- Publication Type:Journal Article
- Keywords:
Biejiajian pill;
hepatocellular carcinoma;
proliferation;
migration;
invasion;
epithelial-mesenchymal transition;
CMTM6
- From:
Chinese Journal of Comparative Medicine
2025;35(7):44-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effects and mechanisms of Biejiajian pill(BJJP)-containing serum on the malignant biological behaviors of hepatocellular carcinoma Huh7 cells.Methods This research knocked down CKLF-like MARVEL transmembrane domain containing 6(CMTM6)expression using a CMTM6-specific small interfering RNA(siRNA).Healthy Sprague-Dawley rats were used to prepare normal rat serum and low-(0.55 g/kg),medium-(1.1 g/kg),and high-(2.2 g/kg)BJJP-containing.Huh7 cells were cultured with normal fetal bovine serum(BC),normal rat serum(NC),and low-,medium-,and high-dose BJJP serum(LBJJP,MBJJP,and HBJJP,respectively).BJJP-containing serum and si-CMTM6 were applied to Huh7 cancer cells,and the proliferation,migration,and invasion abilities were evaluated by CCK-8 and Transwell assays,respectively.Protein expression levels of proliferating cell nuclear antigen(PCNA),epithelial-mesenchymal transition(EMT)markers,and CMTM6 were detected by Western blot.Results CMTM6 knockdown significantly reduced the mRNA and protein expression level of CMTM6 in Huh7 cells(P＜0.05).There were no significant differences between the BC and NC groups in terms of cell proliferation,migration,invasion,expression levels of PCNA,EMT markers,and CMTM6(all P＞0.05).BJJP-containing serum markedly inhibited Huh7 cell proliferation,migration,and invasion(P＜0.05),downregulated PCNA,CMTM6,N-cadherin,and Vimentin expression,and upregulated E-cadherin compared with the NC group(all P＜0.05).CMTM6 knockdown suppressed malignant behaviors,with reduced PCNA,Vimentin,and N-cadherin and elevated E-cadherin expression(all P＜0.05).Conclusions BJJP-containing serum can significantly inhibit Huh7 cell growth,invasion,migration,and EMT progression,potentially mediated via CMTM6 suppression.
