Increased expression of NLRP3 in the hippocampus and prefrontal cortex of mice in a posttraumatic stress disorder model
10.16557/j.cnki.1000-7547.2024.05.003
- VernacularTitle:创伤后应激障碍模型小鼠海马和前额叶皮质的NLRP3表达增加
- Author:
Jixin LI
1
;
Wenlong XING
;
Junxiu JIANG
;
Bin LI
;
Ziwei ZHANG
;
Guiqing ZHANG
Author Information
1. 石河子大学:医学院,石河子 832003
- Publication Type:Journal Article
- Keywords:
posttraumatic stress disorder(PTSD);
NLRP3;
cognitive impairment;
hippocampus;
prefrontal cortex(PFC);
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(5):549-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of NLRP3 inflammatory vesicles in hippocampal tissue and prefron-tal cortex in a mouse model of posttraumatic stress disorder(PTSD).Methods:Male C57BL/6J mice were randomly divided into 2 groups:the control group and the PTSD group.The PTSD group used conditioned foot shock(CF)and single-sustained stress(SPS)to prepare an animal model of PTSD.Anxiety and depression responses of the mouse mod-el were detected by the open field experiment and elevated cross maze test.Memory and memory capacity tests were es-tablished by the darkness-avoidance experimental system.Morphological changes in the hippocampus and prefrontal cor-tex of mice were observed by hematoxylin-eosin staining(HE),and the expression of NLRP3 inflammatory vesicles was detected using Western Blot and immunohistochemical staining.Results:The PTSD mouse group showed decreased body mass,anxiety and depression-like behaviors,and decreased learning and memory abilities compared with the con-trol group(P＜0.05).HE staining showed tissue damage in the hippocampal CA1 region and prefrontal cortex in PTSD mice compared with the control group.Western Blot and immunohistochemical staining showed that after 3 d of PTSD stimulation,hippocampal and prefrontal cortical NLRP3 inflammatory vesicles were activated(P＜0.05).Conclusion:Increased expression of NLRP3 inflammatory vesicles in the hippocampus and prefrontal cortex of PTSD model mice.
