Methodological evaluation of measuring urinary iodine using a fully automated iodine analyzer
10.3760/cma.j.cn231583-20230803-00020
- VernacularTitle:全自动碘分析仪测定尿碘的方法学评价
- Author:
Li XIE
1
;
Junjun ZHAO
;
Yuhua ZHANG
Author Information
1. 濮阳市疾病预防控制中心理化检验科，濮阳 457001
- Publication Type:Journal Article
- Keywords:
Iodine;
Urine;
Methodological evaluation
- From:
Chinese Journal of Endemiology
2024;43(12):1010-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the feasibility of using a fully automated iodine analyzer to measure urinary iodine.Methods:A fully automated iodine analyzer was used to determine the urinary iodine content, a methodological evaluation was conducted on the linear relationship, detection limit, precision, and accuracy (urinary iodine standard substances GBW109m, GBW110e within the range of 0 - 300 μg/L, and GBW111m, GBW112h within the range of 300 - 1 200 μg/L) of the standard curve within the ranged from 0 to 1 200 μg/L (low concentration standard series: 0 - 300 μg/L, high concentration standard series: 300 - 1 200 μg/L). A method comparison experiment with the manual measurement results of arsenic cerium catalytic spectrophotometry was conducted.Results:The fully automated iodine analyzer had a good linear relationship for the determination of urinary iodine within the standard curve ranges of 0 - 1 200 μg/L, with│ r│ > 0.999 0; the minimum detection limit was 3.17 μg/L. Among them, the coefficients of variation for low, medium, and high concentrations of urinary iodine standard substances in the range of 0 - 300 μg/L were 6.79%, 2.53% and 1.20%, respectively; and the recovery rate ranged from 94.8% to 101.2%. The coefficients of variation for low and high concentrations of urinary iodine standard substances within the range of 300 - 1 200 μg/L were 1.21% and 1.62%, respectively; and the recovery rate ranged from 100.6% to 105.1%. The determination results of urinary iodine standard substances GBW109m, GBW110e, GBW111m, and GBW112h were all within the given standard value ± uncertainty range [(69.7 ± 9.0), (210 ± 15), (574 ± 17), (794 ± 28) μg/L]. There was no statistically significant difference between the results obtained by using a fully automated iodine analyzer and the manual measurement results of arsenic cerium catalytic spectrophotometry ( P > 0.05). Conclusion:The fully automated iodine analyzer detection method can be fully automated, with simple and time-saving operation, high system accuracy, and good precision, suitable for large-scale urine iodine sample detection.
