The role of SIRT1/DRP1 in genistein suppressing ox-LDL-induced apoptosis of human umbilical vein endothelial cells
10.20039/j.cnki.1007-3949.2025.08.005
- VernacularTitle:SIRT1/DRP1在染料木素抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞凋亡中的作用
- Author:
Hui ZHOU
1
;
Haixia YU
1
;
Huaping ZHANG
1
Author Information
1. 山西医科大学转化医学研究中心,山西省太原市 030001
- Publication Type:Journal Article
- Keywords:
genistein;
endothelial cell;
apoptosis;
sirtuin 1;
dynamin-related protein 1
- From:
Chinese Journal of Arteriosclerosis
2025;33(8):683-689
- CountryChina
- Language:Chinese
-
Abstract:
Aim To clarify the role of sirtuin 1(SIRT1)/dynamin-related protein 1(DRP1)in genistein inhibi-ting oxidized low density lipoprotein(ox-LDL)-induced apoptosis of human umbilical vein endothelial cells(HUVEC).Methods HUVECs were cultured in vitro.The levels of mitochondrial fission factor(MFF),mitochondrial fission pro-tein 1(FIS1),mitofusin 1(MFN1),mitofusin 2(MFN2),optic atrophy 1(OPA1),Bcl-2,Bax,cytochrome c(Cyt c),apoptotic protease activating factor 1(APAF1),cleaved Caspase-9,cleaved Caspase-3,p-DRP1(Ser616)and acety-lation-DRP1(ac-DRP1),as well as the interaction between p-DRP1 and Bax were examined.Results Compared with ox-LDL treatment,genistein pretreatment suppressed the activity of DRP1 through diminishing acetylation and phos-phorylation,which was associated with activating SIRT1(all P＜0.05).Genistein pretreatment inhibited mitochondrial fission by enhancing MFN1,MFN2 and OPA1,and reducing MFF and FIS1(all P＜0.05).Genistein pretreatment sup-pressed apoptosis through inhibiting the interaction between p-DRP1 and Bax,accompanied with increasing Bcl-2 and de-creasing Bax,Cyt c,APAF1,cleaved Caspase-9 and Caspase-3(all P＜0.05).Conclusion Genistein suppressed ox-LDL-induced mitochondrial fission and apoptosis through activating SIRT1 and inhibiting DRP1 in HUVEC.Therefore,SIRT1/DRP1 had a crucial role in genistein inhibiting ox-LDL-induced apoptosis in HUVEC.
