Distribution and source tracing analysis of drug-resistant bacteria in the environment at pig farms in Shandong Province
10.3969/j.issn.1002-2694.2025.00.103
- VernacularTitle:山东省部分地区养猪厂环境中耐药菌分布及溯源分析
- Author:
Shu-meng YOU
1
;
Yong WANG
;
Da-yang ZOU
;
Hong-bin WANG
;
Jun-zhu BAI
;
Dan-jie ZHANG
;
Liang WEN
;
Yuan-yong XU
;
Wen-yi ZHANG
Author Information
1. 中国医科大学公共卫生学院,沈阳 110122;中国人民解放军疾病预防控制中心,北京 100071
- Publication Type:Journal Article
- Keywords:
whole genome sequencing;
bacterial communities;
bacterial source tracking;
multidrug-resistant bacteria
- From:
Chinese Journal of Zoonoses
2025;41(6):623-628
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the drug resistance and genetic relationships among strains co-existing in animals,the environ-ment,and the living quarters of employees at large-scale pig farms in certain regions of Shandong Province,to provide a scientific ba-sis for elucidating the transmission mechanisms of drug-resistant bacteria through bacterial traceability analysis.Samples were col-lected from two pig farms,and bacteria were isolated and purified.The species of the isolated strains were identified via 16S rRNA gene sequencing.Antimicrobial susceptibility testing was conducted with a VITEK-2 Compact system and the disk diffusion method for strains present in pigs,the environment,and living areas.Furthermore,whole-genome sequencing was performed on the Illumina Miniseq platform to annotate drug resistance genes,and multilocus sequence typing(MLST)and core genome single nucleotide poly-morphism(cgSNP)analyses were used to trace the resistant strains.Three species—Staphylococcus aureus,Pseudomonas aeruginosa,and Bacillus cereus—were isolated and cultured from animals,the environment,and employee living areas,and their distributions were analyzed.These strains exhibited diverse drug resistance spectra and genetic diversity.Additionally,the strains displayed highly consistent resistance profiles,resistance genes,ST types,and SNP loci in pig urine,soil both inside and outside the facility,human drinking water,and the cafeteria and dormitories.Our findings indicated a potential risk of transmission of opportunistic pathogens be-tween the pig farming area and the living quarters.Particular attention should be paid to the environmental transmission of methicillin-resistant Staphylococcus aureus.
