PLIN2 promotes lipid accumulation in THP-1 derived macrophages by upregulating ACSL3 expression
10.20039/j.cnki.1007-3949.2025.07.005
- VernacularTitle:PLIN2通过上调ACSL3表达促进THP-1源性巨噬细胞脂质蓄积
- Author:
Lan LIU
1
;
Li YANG
;
Yuting WANG
;
Xindu LIU
;
Zhonghua YUAN
Author Information
1. 南华大学药学院药理学教研室,湖南省衡阳市 421001
- Publication Type:Journal Article
- Keywords:
adipose differentiation-related protein;
PI3K/Akt signaling pathway;
macrophage;
lipid accumu-lation;
acyl-CoA synthetase long-chain family member 3
- From:
Chinese Journal of Arteriosclerosis
2025;33(7):587-594
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether adipose differentiation-related proteins promote macrophage lipid accu-mulation by upregulating acyl-CoA synthetase long-chain family member 3(ACSL3)expression through PI3K/Akt.Methods The experiments were divided into 24 h group,different PLIN2 expression groups,HA-PLIN2+SC97 group and HA-PLIN2+LY294002 group.Western blot was used to detect the protein expression of PLIN2,Akt,p-Akt and ACSL3 in cells,RT-qPCR was used to detect the mRNA level of PLIN2 in cells,and oil red O was used to observe the degree of lipid accumulation in cells.Results The protein expression levels of Akt,p-Akt and ACSL3 in macrophages overex-pressing PLIN2 were significantly increased(P＜0.05),and p-Akt nuclear translocation was increased,with fluorescence labeling of PLIN2 and Akt overlapping.After adding the PI3K/Akt agonist SC97 to macrophages overexpressing PLIN2,the expression level of ACSL3 significantly increased(P＜0.05),and the degree of intracellular lipid accumulation in-creased;After adding the PI3K/Akt inhibitor LY294002 to macrophages overexpressing PLIN2,the expression level of AC-SL3 significantly decreased(P＜0.05),and the degree of intracellular lipid accumulation decreased.Conclusion PLIN2 upregulates ACSL3 expression through PI3K/Akt,thereby promoting macrophage lipid accumulation.
