Mitochondrial dysfunction mediates pyroptosis in the promoton of inflammatory response in pulpitis
10.3969/j.issn.1001-3733.2025.04.007
- VernacularTitle:线粒体功能障碍介导细胞焦亡促进牙髓炎的炎症反应
- Author:
Maimaitiyiming NUERBIYAMU
1
;
Bingjie LIAN
1
;
Fanyi MENG
1
;
Jin ZHAO
1
Author Information
1. 830000 乌鲁木齐,新疆医科大学第一附属医院(附属口腔医院)牙体牙髓病科,新疆维吾尔自治区口腔医学研究所
- Publication Type:Journal Article
- Keywords:
Pulpitis;
Pyroptosis;
Mitochondrial function;
Cleaved caspase-3
- From:
Journal of Practical Stomatology
2025;41(4):478-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression and regulatory mechanisms of pyroptosis in pulpitis.Methods:The activation sta-tus of cell deathes in pulpitis was analyzed using GSE77459 and GSE92681 datasets.Subsequently,pulp tissue and gingival crevic-ular fluid samples were collected from the subjects with pulpitis(infected)and healthy(non-infected)(n=10)respectively.The levels of GSDMD,NLRP3,caspase-1 and apoptosis proteins were detected by RT-qPCR and Western blot;the levels of IL-1βand IL-18 were assessed by ELISA;and the levels of reactive oxygen species(ROS)and mitochondrial membrane potential were exam-ined by probe method.Results:The levels of pyroptosis in the samples of patients with pulpitis in both GSE77459 and GSE92681 datasets were significantly higher than those in the control group(P＜0.05).RT-qPCR and Western blot analyses revealed that compared to controls,the expression of GSDMD,NLRP3 and caspase-1 in the samples of pulpitis was significantly higher(P＜0.05);the levels of IL-1β,IL-18,ROS and mitochondrial membrane potential in pulpitis were significantly elevated;the levels of eleaved caspase-3,caspase-8 and caspase-9 were significantly increased.Conclusion:The elevated level of pyroptosis in pulpitis that promotes inflammation may be related to mitochondrial dysfunction.
