The effects of paclitaxel combined with Ipatasertib on the proliferation,migration and EMT of oral squa-mous cell carcinoma cells
10.3969/j.issn.1001-3733.2025.04.006
- VernacularTitle:紫杉醇和Ipatasertib联用对OSCC细胞增殖、迁移及上皮间充质转化的影响
- Author:
Pengkun XU
1
;
Di CHE
1
;
Zhehao LI
1
;
Xinpeng DAI
1
;
Jinru WENG
1
;
Jian GUAN
1
Author Information
1. 154002,佳木斯大学附属口腔医院
- Publication Type:Journal Article
- Keywords:
Paclitaxel;
Ipatasertib;
OSCC;
PI3K/AKT;
EMT
- From:
Journal of Practical Stomatology
2025;41(4):473-477
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of paclitaxel combined with Ipatasertib on the proliferation,migration and epitheli-al-mesenchymal transition(EMT)of oral squamous cell carcinoma(OSCC)SCC-9 cells in vitro.Methods:SCC-9 cells were treated with paclitaxel and Ipatasertib repectively and in combination.CCK-8 test and EdU assay were used to detect cell proliferation ca-pacity.Cellular migration was detected by Transwell and scratch method.The protein expressions of E-cad,N-cad,Vimentin,AKT and NF-κB were detected by Western blot.Results:Paclitaxel of 0.5-32 μmol/L combined with Ipatasertib of 2.5 μmol/L(IC50)showed synergistic inhibitory effect on the proliferation of SCC-9 cells,the combination of paclitaxel and ipatasertib more significantly inhibited the migration,down-regulated the protein expression of AKT,NF-κB,N-cad and Vimentin,and up-regulated the protein expression of E-cad.Conclusion:The combination of paclitaxel and Ipatasertib may have synergistic inhibitory effects on the prolif-eration,migration and EMT of OSCC cells,the function may be ralated with the inhibition of PI3K/AKT signaling pathway.
