Effect and mechanism of LINC01088 on proliferation,migration and in-vasion of breast cancer cells
10.3969/j.issn.1009-9905.2025.07.006
- VernacularTitle:LINC01088对乳腺癌细胞增殖、迁移和侵袭的作用及机制
- Author:
Jie LIU
1
;
Hui ZHAO
1
;
Chen ZHAO
1
;
Na-na DONG
1
;
Ning LI
1
;
Hai-ting MAO
1
Author Information
1. 山东大学第二医院 检验医学中心(山东 济南 250033)
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
LINC01088;
Cell cycle;
Epithelial-mesenchymal transition;
PI3K-AKT pathway
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(7):538-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of LINC01088 in breast cancer and its effects on cell prolifera-tion,migration,and invasion.Methods:GEPIA and bc-GenExMiner were used to analyze the correlation between LINC01088 expression levels and clinical characteristics as well as prognosis.The expression of LINC01088 in MCF10A and MDA-MB-231,BT-549,MCF7 were detected by Real-time PCR.The effect of LINC01088 on the biological func-tion of breast cancer cells was examined by overexpressing LINC01088 in breast cancer cells.Cell proliferation was as-sessed using the Incucyte assay,while cell migration and invasion were evaluated using Transwell assays.Western blot-ting was employed to detect the expression of proteins associated with cell proliferation and metastasis.Results:LINC01088 expression was significantly lower in breast cancer tissues compared to normal breast tissues(P<0.05).Data from the bc-GenExMiner database revealed higher LINC01088 expression in HER2 positive patients(P<0.0001),corre-lating with longer overall survival(P=0.0006)and disease-free survival(P=0.0002).The mRNA expression level of LINC01088 in normal breast epithelial cell line was higher than that in breast cancer cell lines(P<0.05).Overexpression of LINC01088 significantly reduced proliferation,migration,and invasion in three breast cancer cell lines(P<0.01).Addi-tionally,LINC01088 upregulated p21 and p27(P<0.01),while downregulating Snail,Slug,PI3K,and phosphorylated Akt(P<0.05).Conclusion:LINC01088 expression was significantly reduced in human breast cancer.In vitro,LINC01088 in-hibited the proliferation,migration,and invasion of breast cancer cells.This effect may be attributed to its role in sup-pressing the PI3K-AKT pathway and epithelial-mesenchymal transition.
