The effects of palmitic acid and static pressure on receptor activator of NF-κB ligand and osteoprotegerin expression in human periodontal ligament cells of different ages
10.3969/j.issn.1001-3733.2025.04.016
- VernacularTitle:棕榈酸和静压力对不同年龄人牙周膜细胞中骨保护素配体和骨保护素表达的影响
- Author:
Yangyang WANG
1
;
Zhanqin CUI
;
Wenjing LI
;
Meng TANG
;
Dandan LI
Author Information
1. 050000 石家庄,河北医科大学第二医院口腔正畸科
- Publication Type:Journal Article
- Keywords:
Palmitic acid;
Human periodontal ligament cells;
Static pressure;
Aging;
Receptor activator of NF-κB ligand;
Osteoprotegerin
- From:
Journal of Practical Stomatology
2025;41(4):531-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of palmitic acid(PA)and static pressure on the expression of receptor activator of NF-κB ligand(RANKL)and osteoprotegerin(OPG)in human periodontal ligament cells(HPDLCs)cultured from the subjects of differ-ent ages.Methods:The effects of PA with the dose(μmol/L)of 10,100,200,250,300,400 and 500 respectively on the prolif-erative activity of HPDLCs cultured in vitro from the subjects aged 12-45 years were determined by CCK-8 method.200 μmol/L PA was selected to treat HPDLCs,and the static pressure of 200 Pa was applied in the in vitro culture.Under different conditions(PA group,static pressure group,PA+static pressure group and blank control group),The expression of RANKL and OPG in HPDLCs was determined by ELISA before and after the treatment at 3,6,12,24,36,48 and 72 h respectively,and the OPG/RANKL ratio was calculated.Results:PA inhibited the proliferation of HPDLCs of all ages(P＜0.05),and the proliferation activity of HPDLCs decreased gradually with the increase of PA concentration,patient age and treatment time.Under static pressure,PA up-regulated the expression of RANKL protein,down-regulated the expression of OPG protein and the ratio of OPG/RANKL(P＜0.05),but RANKL protein expression decreased and OPG protein expression and the ratio of OPG/RANKL increased with age.Conclusion:Combined treatment of PA and static pressure can synergistically promote remodeling of periodontal bone.The expressions of RANKL and OPG is closely related to age of the subjects.
