Investigation of anti-inflammatory effect of titania nanotubes surface through enhancing autophagy under inflammatory microenvironment
10.3969/j.issn.1001-3733.2025.04.002
- VernacularTitle:炎症微环境中二氧化钛纳米管表面通过自噬增强巨噬细胞抗炎效应的研究
- Author:
Kaixiu FANG
1
;
Kexin LIU
;
Wen SONG
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学口腔医院修复科;西安未央德雅正尚口腔门诊部有限责任公司
- Publication Type:Journal Article
- Keywords:
Periimplantitis;
Titania nanotubes;
Autophagy;
Inflammatory microenvironment;
Macrophage polarization
- From:
Journal of Practical Stomatology
2025;41(4):450-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of autophagy in the effects of titanium(Ti)nanotopography on macrophage polarization and inflammatory cytokine secretion under inflammatory microenvironment.Methods:4 groups were set as negative control(NC,on tissue culture plate),polished pure Ti surface(P),30 nm diameter nanotube(NT-30),and NT-30 with 3-Methyladenine(3-MA)treatment.Macrophage cell line RAW264.7 was cultured on different surfaces for 3 d and treated by LPS for 24 h.The autophagy was examined by transmission electron microscopy and Western blot while the cell morphology was observed by scanning electron mi-croscopy.The secretion of inflammatory cytokines was measured by Cytokine Array.Results:Under LPS stimulation,the autophagic degradation activity was significantly enhanced on Ti samples.On NT-30 surface,the autophagosomes formation was significantly in-creased,which was decreased after 3-MA treatment.Meanwhile,cells were obviously elongated on NT-30 surface and returned to spherical shape after 3-MA treatment.In addition,the pro-inflammatory cytokines and anti-inflammatory cytokines were significantly decreased and increased respectively on Ti surfaces,in which the NT-30 surface was the most obvious.After 3-MA treatment,the anti-inflammatory effect of NT-30 surface was inhibited.Conclusion:In inflammatory microenvironment,titania nanotubes surface could enhance autophagy activity to realize anti-inflammatory effect.
