VLCAD inhibits proliferation,invasion,and metastasis of oral squamous cell carcinoma cells by regula-ting GLUT1-mediated aerobic glycolysis
10.3969/j.issn.1001-3733.2025.04.017
- VernacularTitle:VLCAD通过调节GLUT1介导的有氧糖酵解抑制口腔鳞状细胞癌细胞增殖、侵袭和转移
- Author:
Yongqing FU
1
;
Sanhui XU
1
;
Yan ZHAO
1
;
Lili WANG
1
;
Qiuxiang LEI
1
Author Information
1. 054001,邢台市人民医院检验科
- Publication Type:Journal Article
- Keywords:
Oral squamous cell carcinoma;
VLCAD;
GLUT1;
Aerobic glycolysis;
Tumor development
- From:
Journal of Practical Stomatology
2025;41(4):538-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and mechanism of very long chain acyl-CoA dehydrogenase(VLCAD)on prolifera-tion,invasion and metastasis of oral squamous cell carcinoma cells.Methods:The expression levels of VLCAD and glucose trans-porter 1(GLUT1)in human normal oral epithelial cell line(HOEC cells)and human oral squamous cell carcinoma line(SCC25 cells)were determined by Western blot,and the expression levels of VLCAD and GLUT1 in oral squamous cell carcinoma patients and their correlation with pathological stages and prognosis were analyzed in the TCGA database.SCC25 cell lines with VLCAD over-expression and knockdown were constructed and the expression level of VLCAD and GLUT1,cell proliferation,migration,invasion ability,lactate dehydrogenase content and lactate production level were detected respectively.Results:The expression level of VL-CAD in SCC25 cells was significantly lower than that in HOEC cells.The expression level of GLUT1 in SCC25 cells was significantly higher than that in HOEC cells.There was a significant negative correlation between the two expression levels(P＜0.05).VLCAD overexpression significantly inhibited GLUT1 expression and aerobic glycolysis in SCC25 cells,and also inhibited cell proliferation,migration and invasion.VLCAD knockdown effectively reversed the antitumor effects of overexpression mediated by VLCAD(P＜0.05).Conclusion:VLCAD inhibits proliferation,invasion,and metastasis of oral squamous cell carcinoma SCC25 cells by down-regulating GLUT1 and inhibiting its mediated aerobic glycolysis.
