Melatonin synthase regulates osteogenic differentiation of periodontal ligament stem cells by modulating mitochondrial functions
10.3969/j.issn.1001-3733.2025.04.004
- VernacularTitle:褪黑素合成酶调控线粒体功能影响PDLSCs成骨分化的机制研究
- Author:
Jiujiu ZHANG
1
;
Ning ZHANG
;
Chen JIAO
;
Xiaotao HE
;
Xuan LI
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学口腔医院牙周病科
- Publication Type:Journal Article
- Keywords:
AANAT;
ASMT;
Periodontal ligament stem cells;
Mitochondrial function;
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2025;41(4):461-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Aralkylamine N-acetyltransferase(AANAT)and acetylserotonin O-methyltrans-ferase(ASMT)on the osteogenic differentiation of periodontal ligament stem cells(PDLSCs)and the related regulatory mechanisms.Methods:PDLSCs were isolated and cultured from freshly extracted healthy teeth,and transfected with si-AANAT,si-ASMT and negative control(si-NC),respectively.The silencing efficiency on AANAT and ASMT genes and the expression level of osteogenic differentiation related genes of PDLSCs were detected by RT-qPCR.Mitochondrial reactive oxygen species(mtROS)production was measured by flow cytometry and fluorescent probe MitoSOX.The relative content of mitochondrial DNA(mtDNA)was detected by RT-qPCR,the content of ATP was detected by ATP kit,mitochondrial membrane potential(MMP)was measured by JC-1 fluores-cent staining.Results:After transfection of PDLSCs with si-AANAT and si-ASMT,AANAT and ASMT genes in PDLSCs were effec-tively silenced.Suppression of AANAT and ASMT in PDLSCs significantly decreased the mRNA expression levels of OCN,RUNX2 and COL-1.After silencing the expression of AANAT and ASMT,the mtROS production of PDLSCs increased,while the mtDNA,intracellular ATP content and MMP levels decreased significantly.Conclusion:AANAT and ASMT affect the osteogenic differentia-tion potential of PDLSCs possibly via regulating the mitochondrial function.
