Protective effect and mechanism of Ginkgo biloba extracts against cerebral ischemia-reperfusion injury
- VernacularTitle:银杏叶提取物对小鼠脑缺血-再灌注损伤的保护作用及机制研究
- Author:
Ya-ling WANG
1
;
Hai-lin LIU
;
Jun WEN
;
Hui CHEN
;
Peng WANG
;
Jin-jin JI
Author Information
- Publication Type:Journal Article
- Keywords: cerebral ischemia-reperfusion injury; Ginkgo biloba extracts; neurological deficit; Akt; network pharmacology
- From: Journal of Regional Anatomy and Operative Surgery 2025;34(3):212-218
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the mechanism of Ginkgo biloba extracts(GB)in the treatment of cerebral ischemia-reperfusion(CIR)injury based on network pharmacology and animal experiments.Methods The intersection targets of CIR and GB were obtained from TCMSP,GeneCards and other databases.Cytoscape software and Metascape database were used to analyze and map the related targets.The model of transient middle cerebral arterial occlusion(t-MCAO)was constructed in mice by suture method,and the effects of GB on the neurological function of mice after t-MCAO were observed by the neurological deficit score and Morris water maze test.HE staining was used to observe the pathological structural changes of neuron in the hippocampus of mice,and Western blot was used to verify the signal pathways screened by network pharmacology.Results Network pharmacology predicted that GB contained 33 active ingredients,and 116 potential targets of GB in treatment of CIR included Caspase3,Bax,etc.In addition,GB may play a protective role through signaling pathways such as PI3K-Akt and AMPK.Animal experiments showed that GB treatment could significantly improve the neural function and learning spatial memory ability of mice,alleviate the brain histopathological injury,and activate p-Akt/Akt signaling pathway.Conclusion GB has the characteristics of multi-target and multi-pathways therapy for CIR,which may reduce neuronal apoptosis by activating the p-Akt/Akt signaling pathway.
