Exploring mechanism of TLR4/NF-κB-NLRP3 inflammasome signaling pathway in experimental autoim-mune prostatitis rats
10.3969/j.issn.1006-5725.2025.06.004
- VernacularTitle:TLR4/NF-κB-NLRP3炎症小体信号通路在实验性自身免疫性前列腺炎大鼠中的作用机制
- Author:
Liangxi LU
1
;
Hong SHI
;
Zhimin HUANG
;
Jie LU
;
Wenjie WANG
Author Information
1. 广西中医药大学第一附属医院仁爱分院男科(广西 南宁 530001)
- Publication Type:Journal Article
- Keywords:
chronic prostatitis;
chronic pelvic pain syndrome;
NLRP3 inflammasome;
TLR4/NF-κB-NLRP3 inflammasome signaling pathway;
inflammatory factor
- From:
The Journal of Practical Medicine
2025;41(6):800-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective The pathogenesis of EAP in rats based on the TLR4/NF-κB-NLRP3 inflammasome signaling pathway was explored.Methods Randomly divide 12 male SD rats into 4 groups using the number table,namely normal group(N),model group(M),Caspase-1 inhibitor group(Caspase-1),and NLRP3 inhibitor MCC950 group(NLRP3),with 3 rats in each group.After drug intervention,relevant indicators were observed by using HE staining,ELISA,WB methods.Results Compared with the N group,the M group rats had showed significant damage in prostate gland structure and infiltration of inflammatory cells.Compared with group N,the expression of TLR4,P-NF-κB P65,NLRP3,ASC,Cleaced-Caspase-1,Cleaced-IL-1β,and IL-18 proteins in the prostate tissue of group M rats had increased(P<0.01).Compared with group M,the expression of TLR4,P-NF-κB P65,NLRP3,ASC,Cleaced-Caspase-1,Cleaced-IL-1β,and IL-18 proteins in the NLRP3 and Caspase-1 groups had significantly reduced(P<0.01).The serum levels of IL-1 β,IL-6,IL-8,IL-17A,IL-18,IFN-γ,and TNF-α in group M rats had been significantly higher than those in group N(P<0.01).But the serum levels of IL-10 had been slightly lower and no statistical significance.The serum levels of IL-1β,IL-6,IL-8,IL-17A,IL-18,IFN-γ,and TNF-α in group M rats had been lower than those in group N(P<0.01 or P<0.05),the serum IL-10 level had increased(P<0.01).Conclusion The activation of TLR4/NF-κB-NLRP3 inflammasome signaling path-way promotes the occurrence and development of prostatitis in EAP rats.
