Correlation analysis on polymorphisms of multidrug resistance 1 gene and nephrotoxicity induced by cyclosporine in heart transplant recipients
10.3760/cma.j.issn.1008-5734.2014.03.009
- VernacularTitle:多药耐药基因多态性与心脏移植术后环孢素肾毒性的相关性分析
- Author:
Xiujin SHI
1
;
Guoyi WEI
;
Yang LIN
;
Haibo ZHANG
;
Yu ZHONG
;
Yuguo BAI
;
Yang ZHOU
;
Yixin JIA
Author Information
1. 100029,首都医科大学附属北京安贞医院药剂科
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Gene Polymorphism;
Heart transplantation;
Kidney injury
- From:
Adverse Drug Reactions Journal
2014;(3):153-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between polymorphisms of multidrug resistance 1 (MDR1)gene and nephrotoxicity induced by cyclosporine(CsA)in heart transplant recipients. Methods The patients who received cardiac transplantation in Beijing Anzhen Hospital from January 2004 to December 2012,postoperative immunosuppression treatment with CsA,and follow-up for at least 12 months,were selected as subjects. All patients were divided into the kidney injury group and the no kidney injury group according as whether kidney injury occurred after CsA treatment. Leukocyte genomic DNA was extracted from the blood which was taken for CsA concentration monitoring after heart transplant. According to information sites in HapMap database,genotype and allele frequencies of 16 tags of MDR1 SNP were analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry( MALDI-TOF MS). SNP allele frequencies were compared with frequencies obtained from dbSNP database and non-conditional binary logistic regression analysis was used to analyze the correlation between the SNP genetic polymorphisms and kidney injury induced by CsA. Results A total of 65 patients were entered into this study. Of them, 19 patients were in the kidney injury group(17 males and 2 females)with age of 18-59(44 ± 13)years and 46 patients were in the no kidney injury group(39 males and 7 females)with age of 14-71(42 ± 15)years. There was no statistically significant difference in age and gender between the 2 groups( P>0. 05 for all comparisons). According to MALDI-TOF MS detection results,the differences of allele frequencies in 16 tag SNP and corresponding data in dbSNP database,and the differences of allele frequencies in 16 tag SNP between the 2 groups were not statistically significant(all P>0. 05). Based on the non-conditional Logistic regression analysis,there was no significant correlation between genetic frequency of MDR 1 in these 16 Tag SNP and the CsA nephrotoxicity in patients with kidney injury. Conclusion No statistically significant correlation between MDR1 gene polymorphism and nephrotoxicity induced by cyclosporine in heart transplant recipients is found.
