The effects of pepsin on the mucosal epithelium of the laryngopharynx under different pH condition
10.3969/j.issn.1006-7299.2025.02.013
- VernacularTitle:胃蛋白酶在不同pH条件下对咽喉黏膜上皮的作用
- Author:
Yanyan ZHENG
1
;
Donghui YAN
;
Shiyan CHEN
;
Hongxun GONG
;
Xianming CHEN
Author Information
1. 联勤保障部队第九○○医院耳鼻咽喉头颈外科(福州 350025)
- Publication Type:Journal Article
- Keywords:
Laryngopharyngeal reflux diseases;
Pepsin;
pH;
Electron microscopy
- From:
Journal of Audiology and Speech Pathology
2025;33(2):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the damage of pepsin to the mucosal epithelium of the pharynx under a-cidic and non-acidic conditions,and to provide new ideas for the treatment of refractory laryngopharyngeal reflux diseases.Methods A total of 30 healthy guinea pigs were selected and randomly divided into 3 groups(with 10 in each group).The animals in group A were perfused with 0.3％pepsin+hydrochloric acid solution(pH=2),the animals in group B were perfused with 0.3％pepsin+sterilized distilled water(pH=7),and the animals in group C were perfused with sterilized distilled water(pH=7).After 14 days of perfusion,the mucosal tissues of the post-cyclic area were examined by HE staining,immunohistochemistry,and transmission electron microscopy to detect the degree of inflammatory cell infiltration,the expression of occludin and claudin-3 proteins,the degree of epithelial cell gap widening,and also to analyze the changes of cell submicroscopic structure under electron microscopy.Re-sults ① The submucosal leukocyte count was 28.61±7.10 in group A,29.89±7.04 in group B,and 7.81±1.40 in group C.Compared with group C,the leukocyte count was higher in group A and group B(P＜0.05).② Com-pared with group C,both occludin and claudin-3 protein expression were downregulated in group A(P＜0.05),compared with group C,the difference between occludin and claudin-3 protein expression in group B was not statisti-cally significant(P＞0.05).③ Under electron microscopy,the mucosal epithelial cell gap was widened,the mito-chondria was swollen and the cristae was degraded in group A.In group B,there was no widening of the mucosal epithelial cell gap,but the mitochondria in the cytoplasm was swollen and the cristae was degraded,and there was no widening of mucosal epithelial cell gap and the morphological markers of mitochondria were clear in group C.Conclusion Under non-acidic conditions,pepsin still can cause inflammatory changes and mitochondrial damage in the mucosal epithelium of the pharynx.
