Effect of salvianolic acid B on the telomere length in a chronic mild stress rat model of depression
10.3760/cma.j.cn113661-20201005-00426
- VernacularTitle:丹酚酸B对慢性应激抑郁症模型大鼠端粒长度的影响
- Author:
Lan WANG
1
;
Lulu YU
1
;
Jincheng WANG
1
;
Lina JIA
1
;
Fengya ZHEN
1
;
Xueyi WANG
1
;
Cuixia AN
1
Author Information
1. 河北医科大学第一医院精神卫生中心 河北省精神卫生研究所 河北省脑老化与认知 神经科学实验室 河北省脑科学与精神心理疾病重点实验室，石家庄050031
- Publication Type:Journal Article
- Keywords:
Depressive disorder;
Telomere;
Rats
- From:
Chinese Journal of Psychiatry
2021;54(4):280-286
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to explore the change of blood and brain telomere length and the effect of salvianolic acid B on it in a rat chronic mild stress (CMS) model of depression.Methods:A total of 45 Sprague-Dawley (SD) male rats were randomly divided into 5 groups using a random number table, which were the control group, CMS group, fluoxetine group, salvianolic acid B group, and combined medication group, with nine rats in each group. All rats received CMS for 6 weeks. After successfully establishing the depression model (day 22 to day 42 after enrollment), each rat was intraperitoneally injected with 0.9% normal saline, salvianolic acid B (40 mg·kg -1·d -1), and/or fluoxetine (20 mg·kg -1·d -1) respectively according to its belonging group. The body mass of each group was tested before admission and every weekend after admission. The depressant-like behaviors were evaluated using sucrose preference test (SPT) and forced swimming test (FST) before (day-1 and-2) and after the depression model established (day 21 and 22) and after treatment (day 42 and 43) respectively. The relative telomere length in the blood, hippocampus, cortex, and cerebellum were analyzed using RT-PCR, respectively. Two-factor repeated analysis of variance was used to analyze the differences in body mass, sucrose preference, and immobility time among the five groups. The Kruskal-Wallis H test was used to compare the relative telomere length among the five groups. The Spearman's rank correlation test was used to analyze the correlation between the telomere length and body mass, sucrose preference, and immobility time at different body parts. Results:After 3 weeks of intervention, compared with those in the CMS group, rats in the salvianolic acid B group, fluoxetine group, and combination medication group showed increased body mass ( P=0.049, P=0.008, P=0.036), raised sucrose preference value ( P=0.089, P=0.094, P=0.041), and shortened forced swimming immobility time (all P<0.01). Compared with the control group, the CMS group presented statistically significantly shortened blood relative telomere length (8.53 (3.95) vs. 0.12 (0.23), P<0.01, Bonferroni adjusted P=0.002). The relative telomere length of the bilateral hippocampus, cortex, and cerebellum did not significantly differ between the control group and the CMS group. Compared with the CMS group, the relative telomere length in the salvianolic acid B group ( P=0.005, Bonferroni adjusted P=0.051), fluoxetine group ( P<0.01, Bonferroni adjusted P=0.005), and combined medication group ( P=0.001, Bonferroni adjusted P=0.007) increased significantly in the blood sample but not in different brain regions. Spearman correlation analysis showed that there was no correlation between the telomere length of different body parts and the body weight, sucrose preference value, and forced swimming immobility time that assessed after the intervention. Conclusion:The shortened telomeres length in the peripheral blood in depression model rats cannot indicate the change of telomere length in the brain. Salvianolic acid B can block the shortening of blood telomere length in depression model rats, with comparable efficacy of fluoxetine.
