The effect of ganglioside on the expression of Caspase-3, Bax, Bcl-2 in the hippocampus of mice with chronic alcoholism
10.3760/cma.j.cn113661-20200619-00284
- VernacularTitle:神经节苷脂对慢性酒精中毒小鼠海马Caspase-3、Bax、Bcl-2蛋白表达水平的影响
- Author:
Chuanyi KANG
1
;
Na ZHAO
;
Mavlikhanova ASIYA
;
Jian HU
Author Information
1. 哈尔滨医科大学附属第一医院精神卫生中心 150001
- Publication Type:Journal Article
- Keywords:
Alcoholism;
Gangliosides;
Blotting, Western;
Caspase-3;
Bax;
Bcl-2
- From:
Chinese Journal of Psychiatry
2020;53(5):426-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of ganglioside (GM1) on the expression of Caspase-3, Bax and Bcl-2 in hippocampus of mice with chronic alcoholism.Methods:Forty male Balb/c mice were randomly allocated into chronic alcoholism group ( n=10), control group ( n=10), low dose GM1 treatment group ( n=10) and high dose GM1 treatment group ( n=10). The mouse model of chronic alcoholism was established by intragastric administration of alcohol in the chronic alcoholism group (the concentration of alcohol solution gradually increased from 5% to 35%), mice in the control group were given the same amount of normal saline, while those in the low and high dose treatment groups were given intraperitoneal injection of GM1 (10, 30 mg/kg per day) after intragastric administration of alcohol. Four weeks after oral administration, alcohol preference test and behavior test were performed to determine the expression levels of Caspase-3, Bax and Bcl-2 in hippocampus. One-way ANOVA was used for comparison between groups, and Bonferroni method was used for pairwise comparison. Results:There were significant differences of alcohol consumption rate among the four groups ( F=630.83， P<0.05).The consumption rate of control group (18.9%±2.2%) and high dose GM1 treatment group (50.9%±3.2%) was lower than that of chronic alcoholism group (56.5%±3.0%).Similarly, there were also significant differences of behavior test result among the four groups ( F=379.52, 7001.18, 33.87, 305.06, all P<0.05). The results in chronic alcoholism group were lower than that of control group, and high dose GM1 treatment group were better than those in chronic alcoholism group ( P<0.05). Results of Western Blot showed that there were significant differences in the expression of Caspase-3, Bax and Bcl-2 proteins among the four groups ( F=12.42, 14.07, 242.37, all P<0.05). Compared to chronic alcoholism group, the expression of Caspase-3 protein and Bax protein in hippocampus of control group and high dose GM1 treatment group decreased significantly, while the expression level of Bcl-2 protein in hippocampus of control group, high dose GM1 treatment group and low dose GM1 treatment group was significantly higher than that of chronic alcohol intoxication group ( P<0.05). Conclusions:Gangliosides can decrease the expression of Caspase-3 and Bax protein in the hippocampus of chronic alcoholism mice, and increase the expression of Bcl-2 protein.
