Active systemic allergic reaction induced by breviscapine injection in guinea pigs
10.3760/cma.j.issn.1008-5734.2015.03.010
- VernacularTitle:注射用灯盏花素致豚鼠主动全身过敏反应的研究
- Author:
Li ZHOU
1
;
Yongwei LUO
;
Yong WANG
;
Juan JIANG
;
Yuling JIA
;
Bo GUI
;
Liming CHONG
;
Zuyue SUN
Author Information
1. 200032上海市计划生育科学研究所/中国生育调节药物毒理检测中心
- Publication Type:Journal Article
- Keywords:
Breviscapine;
Anaphylaxis;
Dose-response relationship,drug;
Reaction time
- From:
Adverse Drug Reactions Journal
2015;(3):204-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationships between active systemic allergic reaction induced by breviscapine injection and the drug dose and the sensitization time. Methods Active systemic allergic reaction in guinea pigs was used as experimental method. Forty-eight guinea pigs were divided into 6 groups according to random number table:breviscapine injection 1,5,25 and 50 mg/kg group( the breviscapine injection group 1,2,3,4),0. 9% sodium chloride injection group( the negative control group)and bovine serum albumin( BSA)control group( the positive control group). Each group comprised 8 guinea pigs. Sensitization:the guinea pigs in group 1 to 4 were given the breviscapine injection at doses of 1,5,25 and 50 mg/kg(0. 5 ml)by intraperitoneal injection every other day for three times,respectively. The the guinea pigs in the negative control group and the positive control group were given 0. 9% sodium chloride injection(0. 5 ml)and bovine serum albumin( BSA)20 mg/kg by intraperitoneal injection every Excitation:the sensitized guinea pigs in each group were divided into 2 subgroups,each subgroup comprised 4 guinea pigs. On the 14 and 21 days after the last sensitization,the guinea pigs in breviscapine 1 to 4 subgroups received 2 times of breviscapine injection intravenously(1. 0 ml),respectively. The guinea pigs in the negative and the positive control subgroups received 2 times of control articles intravenously,respectively. The symptoms of anaphylactic reaction( pilo-erection,shiver, scratching nose,sneeze,cough,vomiturition,cyanosis,dyspnea,urinary and fecal incontinence,instability of gait or tumble,convulsion or hyperspasmia,shock and death ) were observed every day during the sensitization phase. The guinea pigs′ reactions which appeared in 30 min after intravenous injections were observed and the occurrence time of allergic symptoms/signs were recorded attentively. The anaphylactic reaction was determined according to the Chinese Pharmacopoeia′s allergic reaction test. Results The guinea pigs in 6 groups did not show any allergic symptoms in the sensitization phase. Within 30 min in fourteenth days′excitation,2,4,4,4 guinea pigs developed allergic reactions in the breviscapine 1 to 4 groups,respectively. But none of them were identified as allergic reaction. The 4 guinea pigs in the positive control group were judged to have positive allergic reaction. Within 30 min of 21 days′excitation,4、3、4、4 guinea pigs developed allergic reactions in the breviscapine 1 to 4 groups,respectively. Only one guinea pig in the breviscapine 4 group was identified as allergic reaction. The 4 guinea pigs in the positive control group were all judged positive allergic reaction. The guinea pigs in the negative control group did not develop any allergic reactions during the 2 times of excitation. The occurrence time of allergic reactions in the breviscapine groups( within 20 min after excitation)on 21 days′excitation was shorter than those( within 25 min after excitation)occurred on 14 days′ excitation,but the difference was not statistically significant. Conclusions The active systemic allergic reactions induced by breviscapine injection are associated with drug dose and sensitization time. The larger dose and longer sensitization time can increase the risk of allergic reaction and shorten the latency of allergic reaction.
