Lactylation for hepatocyte proliferation and liver regeneration after hepatectomy
- VernacularTitle:肝细胞乳酸化修饰促细胞增殖和部分肝切除术后肝再生
- Author:
Rong-cheng XIAO
1
;
Can WEI
;
Si-yuan HAN
;
Qi ZHAO
;
Ran-ran WANG
;
Hua WANG
;
Ling FANG
Author Information
- Publication Type:Journal Article
- Keywords: liver regeneration; partial hepatectomy; lactylation; lactate; oxamate; hepatocyte proliferation
- From: Chinese Pharmacological Bulletin 2025;41(8):1445-1453
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the changes in hepatic lactylation during liver regeneration and its impact on regeneration.Methods A partial hepatectomy(PHx)mouse model was used to study liver regenera-tion.Sodium oxamate was administered intraperitoneal-ly to inhibit lactate dehydrogenase,and blood and liver tissues were collected at different time points post-sur-gery.The histopathological status was observed using HE staining.Proliferating cell nuclear antigen(PC-NA)levels were detected by immunohistochemistry.Lactylation was assessed using immunofluorescence.Liver LDH enzyme activity,lactate levels and serum alanine aminotransferase(ALT)and aspartate trans-aminase(AST)levels were measured using assay kits.Results After PHx,the liver volume of mice gradual-ly increased,returning to preoperative size on day 7.PCNA levels peaked at 48 hours post-surgery.Liver tissue lactate levels increased to approximately 1.5 times the preoperative level at 12 hours post-surgery and remained elevated until day 7.The lactylation lev-el in hepatocytes peaked at 24 hours post-surgery,gradually declined after 48 hours,and returned to pre-operative levels on day 7.Compared to the PHx group,the sodium oxamate(750 mg·kg-1)+PHx group showed significantly reduced lactylation levels in hepa-tocytes and a smaller liver regeneration volume on day 7.Conclusion Lactylation regulates hepatocyte pro-liferation and promotes liver regeneration after PHx.
