Role of miR-130b-3p/USP47/NLRP3 inflammasome in airway remodeling in asthma
- VernacularTitle:miR-130b-3p靶向USP47去泛素化NLRP3在哮喘气道重塑中的作用机制
- Author:
Chang-lin QUAN
1
;
Zhi-guang WANG
;
Qiao-yun BAI
;
Ning-po DING
;
Yi-lan SONG
;
Guang-hai YAN
Author Information
- Publication Type:Journal Article
- Keywords: asthma; miR-130b-3p; USP47; NLRP3 inflammasome; airway remodeling
- From: Chinese Pharmacological Bulletin 2025;41(8):1500-1508
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the role of miR-130b-3p in regulating the USP47/NLRP3 inflammasome in airway remodeling associated with asthma and to explore its potential therapeutic value in asthma treat-ment.Methods An OVA-induced asthma mouse mod-el was established,and intervention with miR-130b-3p agomir was performed.Histological staining,quantita-tive real-time PCR,Western blot,immunofluorescence and flow cytometry were used to analyze the effects of miR-130b-3p on the expression of USP47,NLRP3,and related inflammatory factors,as well as the inflamma-some activity.Results miR-130b-3p was significantly downregulated in asthmatic mice,and its intervention significantly inhibited airway epithelial damage,inflam-matory cell infiltration,and collagen deposition.Addi-tionally,miR-130b-3p targeted USP47 and indirectly suppressed NLRP3 expression,leading to reduced in-flammasome activity and alleviated asthma-related in-flammatory responses.Conclusion miR-130b-3p re-duces asthma-related inflammatory responses by down-regulating USP47 expression and indirectly inhibiting NLRP3 inflammasome activity.
