Development and application of a real-time PCR detection method for Helicobact-er pylori in canines
10.16303/j.cnki.1005-4545.2025.06.13
- VernacularTitle:犬幽门螺杆菌荧光定量PCR检测方法的建立及应用
- Author:
Qianle REN
1
;
Zhanqing YANG
;
Jinjun LI
;
Bin GUO
;
Xiaoqiong LI
Author Information
1. 吉林大学动物医学学院,吉林长春 130062;浙江省农业科学院食品科学研究所农产品质量安全危害因子与风险防控国家重点实验室,浙江 杭州 310021
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
dog;
epidemiological investigation;
qPCR
- From:
Chinese Journal of Veterinary Science
2025;45(6):1202-1209
- CountryChina
- Language:Chinese
-
Abstract:
Helicobacter pylori(H.pylori)can colonize the gastric mucosa of humans and animals,leading to persistent infection,chronic inflammation,and tissue damage.To understand the preva-lence of H.pylori in dogs,this study designed specific primers targeting a unique region of the H.pylori virulence gene vacA and established a SYBR Green Ⅰ-based real-time PCR(qPCR)method.The specificity,sensitivity,and repeatability of this method were evaluated.Using the es-tablished method,alongside conventional PCR and H.pylori stool antigen(HpSA)detection,we conducted an epidemiological survey of H.pylori infection in pet dogs in urban Hangzhou.The re-sults showed that the standard curve demonstrated a good linear relationship within the concentra-tion range of 3.26×106 to 3.26×101 copies/μL.The method specifically detected H.pylori,with a minimum detection limit of 3.26×101 copies/μL.The intra-and inter-assay coefficients of variation ranged from 0.84％to 1.87％and 0.96％to 1.93％,respectively.Applying this method to canine fe-cal samples collected in Hangzhou from 2023 to 2024 revealed an overall H.pylori positive rate of 25.21％.In comparison,the detection rates by conventional PCR and HpSA were 10.68％and 8.97％,respectively.These results indicate that the established qPCR method is suitable for detec-ting H.pylori infection in dogs,providing a rapid,sensitive,specific,and reproducible quantitative detection method.Additionally,this study confirms the significant prevalence of H.pylori infection in household dogs.Given the widespread dissemination of H.pylori and its potential zoonotic risk,So recommend implementing risk communication and ongoing regional monitoring programs to protect the health of both humans and pets.
