Distribution of street rabies virus in salivary glands of dogs and mice following ex-perimental infection
10.16303/j.cnki.1005-4545.2025.06.10
- VernacularTitle:人工感染狂犬病病毒街毒在犬和小鼠唾液腺中的分布
- Author:
Chongyang WANG
1
;
Danwei ZHANG
1
;
Yannan ZHANG
1
;
Yuping LIU
1
;
Xin GUO
1
;
Yidi GUO
1
;
Maolin ZHANG
1
Author Information
1. 吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室/人兽共患病研究教育部重点实验室/人兽共患病研究所,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
street rabies virus;
sublingual gland;
submandibular gland;
parotid gland;
PB4
- From:
Chinese Journal of Veterinary Science
2025;45(6):1178-1185
- CountryChina
- Language:Chinese
-
Abstract:
The rabies virus(RABV)that causes rabies mainly attacks the peripheral and central nervous systems.In the later stages of infection,it is scattered in the salivary glands and transmit-ted to other susceptible animals through infectious saliva.To study dispersion of the RABV in the three pairs of salivary gland tissues,the street strain PB4 of the RABV was inoculated into 21-day-old female mice through the hind limb muscles.During the moribund stage of the mice,the sublin-gual gland,submandibular gland and parotid gland were collected,respectively.The TCID50 titer of RABV in the three kinds of glands of the mice and the copy number of the RABV N gene were de-tected,and RABV in different salivary glands was observed by immunofluorescence.The results showed that PB4 was dispersed in all three kinds of salivary glands of the mice,with the largest a-mounts in the parotid gland,followed by the submandibular gland,and the lowest amount in the sublingual gland.Three-month-old dogs were inoculated with PB4 through the cranial cavity,and saliva were collected every 12 h after inoculation.The saliva samples were detected by TCID50 and RT-qPCR.And during the moribund stage of the dogs when the disease occurred,the three pairs of salivary glands were collected.Through the determination of the TCID50 titer,RT-qPCR and immu-nofluorescence detection,it was demonstrated that among the three different salivary glands of the dogs,the largest amount of PB4 was found in the parotid gland and the lowest in the sublingual gland.Our results in mice and dogs clearly proved that the parotid gland was consistently found to exhibit the highest content of street RABV among the three major salivary glands,which could en-rich experimental data for analyzing the dispersion of RABV in the salivary glands and interpreta-tion of the intermittent secretion of saliva in clinically rabid dogs.
