Lycium barbarum polysaccharide inhibits iron death through SLC7A11/GPX4 pathway to reduce cerebral ischemia-reperfusion injury in mice
10.16557/j.cnki.1000-7547.2024.05.011
- VernacularTitle:枸杞多糖通过SLC7A11/GPX4通路抑制铁死亡减轻小鼠脑缺血再灌注损伤
- Author:
Zhenghao QIU
1
;
Yaping MA
;
Changsheng MA
;
Bo HAN
;
Shuchen MENG
;
Min BAI
;
Maotao HE
Author Information
1. 山东第二医科大学:基础医学院,潍坊 261021
- Publication Type:Journal Article
- Keywords:
Lycium barbarum polysaccharide(LBP);
ischemia-reperfusion(I/R);
solute carrier family 7 member 11(SLC7A11);
glutathione peroxidase 4(GPX4);
ferroptosis;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(5):607-612
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether Lycium barbarum polysaccharides(LBP)can inhibit ferroptosis through the solute carrier family 7 member 11(SLC7A11)/glutathione peroxidase 4(GPX4)pathway and thus alleviate cerebral ischemia-reperfusion(I/R)injury.Methods:The mouse model of cerebral I/R injury was established by mid-dle cerebral artery occlusion(MCAO)and treated with Lycium barbarum polysaccharides(LBP).The mice were ran-domly divided into three groups:sham group,I/R group,and I/R+LBP group.2,3,5triphenyltetrazolium chloride(TTC)staining was used to observe the volume of cerebral infarcts;Neurological function was assessed by neurological deficit score;The superoxide dismutase(SOD),glutathione(GSH),malondialdehyde(MDA),and Fe2+detection kit to detect the changing levels of SOD,GSH,MDA,and Fe2+;Western Blot detects the expression of ferroptosis-related keys GPX4,SLC7A11,and Nrf2.Results:LBP can reduce the cerebral infarction volume and improve the neurological function.LBP increases the content of SOD and GSH and reduces the content of MDA in I/R injured mice.Western Blot results show that the expression levels of ferroptosis-related proteins GPX4,SLC7A11,and Nrf2 in the brain tissue of the I/R group were significantly lower than those of the Sham group.After LBP treatment,the levels of GPX4,SLC7A11,and Nrf2 in the LBP+I/R group were significantly higher than those of the I/R group.Conclusion:LBP at-tenuates cerebral I/R injury by inhibiting ferroptosis through the SLC7A11/GPX4 pathway.LBP has a neuroprotective effect on cerebral I/R injury and is a potential neuroprotective agent.
