The effects of scutellarin on SIRT1/NF-κB signaling pathway in microgial of cerebral ischemic rat

VernacularTitle:灯盏乙素对脑缺血大鼠小胶质细胞SIRT1/NF-κB通路的影响
Author: Dongyao XU ¹ ; Zhaoda DUAN ; Yujia YANG ; Yingqi PENG ; Yuke WU ; Li YANG ; Chunyun WU
Author Information

1. 昆明医科大学:基础医学院人体解剖与组织胚胎学系,昆明 650500
Publication Type:Journal Article
Keywords: ischemia stroke; scutellarin; middle cerebral artery occlusion(MCAO); SIRT1/NF-κB pathway; macroglia(MG)
From: Chinese Journal of Neuroanatomy 2024;40(5):581-587
CountryChina
Language:Chinese
Abstract: Objective:Investigating the effect of scutellarin on the expression of silent information regulator 1/nucle-ar factor κB(SIRT1/NF-κB)signaling pathway in microglia of cerebral ischemic rat.Methods:Male SD rats were de-vided into sham group,middle cerebral artery occlusion group(MCAO group),MCAO+scutellarin treatment group(MCAO+S group)randomly.Western Blot and immunofluorescence were used to detect the expression of SIRT1 and NF-κB(p65)and phosphorylation of NF-κB(p-p65)of ischemic cortex of rats.Results:Results of Western Blot and immunofluorescence showed that scutellarin pretreatment significantly increased the expression of SIRT1 and reduced phosphorylation of NF-κB p65(P＜0.05).Conclusion:Scutellarin could regulate SIRT1/NF-κB signaling pathway in microglia of cerebral ischemic rat.