Effects of Lycium barbarum glycopeptide on testes of senescent rats induced by D-galactose
10.16303/j.cnki.1005-4545.2025.06.25
- VernacularTitle:枸杞糖肽对D-半乳糖致衰老大鼠睾丸的影响
- Author:
Wenxin MA
1
;
Chang LIU
;
Jing PU
;
Hongmei LI
;
Li YANG
;
Xiangdong ZHU
;
Dongmei CHEN
;
Huiming MA
Author Information
1. 宁夏医科大学生育力保持教育部重点实验室,宁夏银川 750004
- Publication Type:Journal Article
- Keywords:
Lycium barbarum glycopeptide(LbGp);
aging;
Keap1-Nrf2 signaling pathway;
testis;
rat
- From:
Chinese Journal of Veterinary Science
2025;45(6):1296-1304
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the protective effect of Lycium barbarum glycopeptide(LbGp)on testicu-lar injury induced by D-galactose(D-gal)in aging rats,male SD rats were randomly divided into 6 groups:the blank control group(Control),aging model group(Model),positive control group(β-nicotinamide mononucleotide,NMN),low dose LbGp group(LLbGp),medium dose LbGp group(MLbGp)and high dose LbGp group(HLbGp).The testicular mass of rats was counted,the morphological changes of testicular tissue were observed by hematoxylin-eosin(HE)staining,the testicular senescence was detected by β-galactosidase(SA-β-gal)staining,and the levels of testos-terone(T),luteinizing hormone(LH)and follicle stimulating hormone(FSH)in serum were de-tected.The levels of oxidative factors such as glutathione peroxidase(GSH-Px),superoxide dis-mutase(SOD),malondialdehyde(MDA),catalase(CAT)and inflammatory factors such as tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in rat tissues were measured.TUNEL staining was used to detect the apoptosis of testicular cells,epididymal sperm quality,and the expression of Keap1,Nrf2 and Nqo1 mRNA were analyzed.The results showed that compared with the Model group,the testicular coefficient of Lycium barbarum glycopeptide rats in MLbGp and HLbGp groups increased(P＜0.01),the level of T in serum and sperm quality increased(P＜0.05),the structural degeneration and aging of testicular tissue decreased(P＜0.01),the level of antioxidant factors increased,and the levels of inflammatory factors and apopto-sis decreased(P＜0.05).The expression of Keap1 decreased significantly(P＜0.01),while the ex-pression of Nrf2 and Nqo1 mRNA increased(P＜0.05).The above results indicate that Lycium barbarum glycopeptide can improve D-gal-induced testicular senescence,attenuate oxidative stress,reduce inflammatory response,decrease apoptosis,and exert a protective effect on testicular injury in rats due to senescence through the Keap1-Nrf2 signaling pathway.
