Daidzein ameliorate LPS-induced inflammatory response and tight junction injury in mammary epithelial cells of dairy cows
10.16303/j.cnki.1005-4545.2025.06.23
- VernacularTitle:大豆苷元改善LPS诱导的奶牛乳腺上皮细胞炎症反应与紧密连接损伤
- Author:
Xiaoxuan WANG
1
;
Xin RAN
1
;
Kefei LI
1
;
Yu CAO
1
;
Wenjin GUO
1
;
Shoupeng FU
1
;
Juxiong LIU
1
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
Daidzein;
LPS;
mammary epithelial cells;
NF-κB;
tight junctions
- From:
Chinese Journal of Veterinary Science
2025;45(6):1280-1287,1295
- CountryChina
- Language:Chinese
-
Abstract:
Mastitis is one of the common and prevalent diseases in dairy cows,and the natural prod-uct daidzein is a kind of natural flavonoids with a wide range of pharmacological and anti-inflam-matory effects.However,the effect of daidzein on mastitis in dairy cows has not been reported.Therefore,in this study,we explored the effects of daidzein on LPS-induced inflammatory response and tight junction damage in dairy mammary epithelial cells Firstly,we pretreated the MAC-T cell line using different concentrations of daidzein,and it was clarified that daidzein below 200 μmol/L had no effect on the cell activity.Next,we examined the production of pro-inflammatory mediators in LPS-stimulated MAC-T cell lines using qRT-PCR,and clarified that daidzein could reduce the production of pro-inflammatory mediators in a concentration-dependent manner.Subsequently,af-ter the expression of Occludin,Claudin3 and ZO-1 was detected by immunofluorescence and West-ern Blot,it was clear that daidzein could alleviate MAC-T cell intercellular tight junction injury.Fi-nally,it was demonstrated that daidzein significantly inhibited LPS-induced activation of the NF-κB signaling pathway within MAC-T using network pharmacological analysis and Western Blot.The above results suggest that daidzein can inhibit LPS-induced inflammatory response and tight junc-tion damage in mammary epithelial cells of cows by suppressing the activation of NF-κB signaling pathway.The present study provides a theoretical basis for the alleviation of mastitis by natural products and further expands the pharmacological effects of daidzein.
