miR-146a chitosan nanoparticles preparation and protective effects on acute liver cell injury induced by LPS
10.16303/j.cnki.1005-4545.2025.06.20
- VernacularTitle:miR-146a壳聚糖纳米粒的制备及其对LPS诱导的急性肝细胞损伤的保护作用
- Author:
Saining WANG
1
;
Huifang BAI
;
Ning JIANG
;
Qianqian DANG
;
Fengying YIN
;
Lin SUN
;
Xuelin WANG
Author Information
1. 吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室/人兽共患病研究教育部重点实验室/人兽共患病研究所,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
chitosan;
galactose;
arginine;
miR-146a;
acute liver cell injury
- From:
Chinese Journal of Veterinary Science
2025;45(6):1260-1267
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the characteristics of chitosan-loaded miR-146a nanoparti-cles and explore their protective effect against acute liver cell injury induced by LPS in vitro.Galac-tosylated arginine chitosan(GCA)nanoparticles were successfully synthesized,prepared and used to further prepare GCA-miR-146a nanoparticles with different mass ratios by ion cross-linking method.The binding efficiency of miR-146a by GCA nanoparticles was detected by gel retardation experiment.Particle size,morphology and potential were observed and detected by transmission e-lectron microscopy and Zetasizer NanoZS90 respectively.The cytotoxicity of GCA nanoparticles on HepG2 cells was detected by CCK-8 kit.Cellular uptake was assayed by fluorescence microscopy to select the optimum ratio for the further study.The LPS induced acute liver cell injury were evalua-ted by real-time fluorescence quantitative PCR.The results showed that the mass ratio of GCA-miR-146a was 5∶1.Encapsulation efficiency and drug loading efficiency of the nanoparticles reached 97.11％and 20.08％,respectively.The 100 nm nanoparticles with 1.15 mV surface charge showed uniform morphology.The GCA-miR-146a nanoparticles had no cytotoxicity and had high transfection efficiency on HepG2 cells.The study demonstrated that GCA-miR-146 ananoparticles could significantly increase the expression of miR-146a in HepG2 cells and reduce the mRNA ex-pression levels of IL-6 and TNF-α in vitro.The prepared GCA nanoparticles loaded with miR-146a had high loading efficiency and could protect LPS-induced acute liver cell injury in vitro.
