Curcumin alleviates oxygen and glucose deprivation-induced damage to brain microvascular endothelial cells through the PINK1/Parkin pathway
10.16557/j.cnki.1000-7547.2024.05.009
- VernacularTitle:姜黄素通过PINK1/Parkin通路减轻OGD/R诱导的脑微血管内皮细胞损伤
- Author:
Yuqun QIN
1
;
Jianjun ZHAO
;
Hui WANG
;
Litao WEN
;
Yani HU
;
Rao FU
Author Information
1. 广东食品药品职业学院护理学院,广州 510520
- Publication Type:Journal Article
- Keywords:
curcumin;
brain microvascular endothelial cells(BMECs);
oxygen-glucose deprivation/reperfusion(OGD/R);
mitophagy;
PINK1/Parkin pathway
- From:
Chinese Journal of Neuroanatomy
2024;40(5):593-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism by which curcumin activates the PINK1/Parkin pathway to enhance mitophagy and ameliorate endothelial cell damage induced by oxygen-glucose deprivation/reperfusion(OGD/R).Methods:An OGD/R injury model was established in mouse brain microvascular endothelial cells(BMECs).En-dothelial cells were treated with different concentrations of curcumin.The effects of curcumin on mitophagy in BMECs were assessed using immunofluorescence and transmission electron microscopy.Cell proliferation,injury,and barrier integrity were evaluated using a Cell Counting Kit-8(CCK-8),lactate dehydrogenase(LDH)assay,and transendothe-lial electrical resistance(TEER)measurement,respectively.Western Blot was used to detect the effects of curcumin on the expression of PINK1/Parkin pathway proteins(LC-3 Ⅱ/Ⅰ,PINK1,Parkin and p62).Additionally,cells were co-treated with Mdivi-1 to further confirm the role of mitophagy in curcumin's protective effects against OGD/R injury.Results:Curcumin treatment significantly promoted mitophagy,increased the expression of LC-3 Ⅱ/Ⅰ,PINK1,and Par-kin proteins(P＜0.001),and decreased the expression of p62 protein(P＜0.001).In the OGD/R model,curcumin significantly enhanced cell proliferation rates(P＜0.001),reduced LDH release(P＜0.001),and improved TEER values(P＜0.001).However,these protective effects of curcumin were reversed by the mitophagy inhibitor Mdivi-1.Conclusion:Curcumin effectively alleviates cell damage in BMECs under OGD/R conditions by enhancing mitophagy through the activation of the PINK1/Parkin pathway.
