rhCol Ⅲ hydrogel with cell-free fat extract aids spinal cord injury repair in rats
10.16557/j.cnki.1000-7547.2024.05.006
- VernacularTitle:负载无细胞脂肪提取物的rhCol Ⅲ水凝胶促进大鼠脊髓损伤修复
- Author:
Biao YANG
1
;
Zhonghuan WU
;
Tingdong LI
;
Fugui JIANG
;
Junfeng WU
Author Information
1. 黔东南苗族侗族自治州人民医院:骨科,凯里 556000
- Publication Type:Journal Article
- Keywords:
spinal cord injury(SCI);
cell-free fat extract(CEFFE);
recombinant human collagen Ⅲ protein(RhCol Ⅲ);
neural regeneration;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(5):572-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the repair impact of recombinant human collagen Ⅲ(rhCol Ⅲ)hydrogel with sus-tained cell-free fat extract(CEFFE)release on spinal cord injury(SCI).Methods:The rhCol Ⅲ hydrogel was com-bined with CEFFE to create a composite scaffold capable of releasing CEFFE.Rats with SCI hemicut models were divid-ed into three groups:SCI,rhCol Ⅲ,and rhCol Ⅲ/CEFFE.Results:The rhCol Ⅲ/CEFFE hydrogel features a three-dimensional(3D)porous structure and suitable elasticity,enabling continuous CEFFE release.This group demonstra-ted notable motor function improvement and more complete spinal cord tissue continuity compared to other greups.The enzyme-linked immunosorbent assay(ELISA),Luxol fast blue staining(LFB),and immunofluorescence results dem-onstrated that RhCol Ⅲ/CEFFE hydrogel inhibits inflammatory factors,encourages microglia M2 transformation,reduces glial scar formation,and promotes neuronal differentiation,axonal regeneration,and myelin sheath formation.Conclusion:The rhCol Ⅲ/CEFFE hydrogel reshapes the inhibitory microenvironment,supports nerve regeneration and neural network formation,and aids in neural function recovery.
