Deferasirox inhibits lipid peroxidation and ferroptosis in human retinal endothelial cells
10.3760/cma.j.cn511434-20240701-00243
- VernacularTitle:地拉罗司抑制人视网膜血管内皮细胞脂质过氧化和铁死亡
- Author:
Yan LI
1
;
Zixuan CHENG
;
Ting LUO
;
Hongbin LYU
Author Information
1. 西南医科大学附属医院眼科, 泸州 646000
- Publication Type:Journal Article
- Keywords:
Retinal vascular endothelial cell;
Diabetic retinopathy;
Deferasirox;
Ferroptosis;
Lipid peroxidation;
Cell experiment
- From:
Chinese Journal of Ocular Fundus Diseases
2024;40(12):947-953
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe and preliminarily explore the effects of Deferasirox (DFX) on lipid peroxidation and ferroptosis in human retinal endothelial cells (HREC).Methods:A cell experimental study. Divided the in vitro cultured HREC into normal glucose (NG) group, high glucose (HG) group, NG+DFX group, HG+DFX group, NG+DFX+ferric ammonium citrate (FAC) group, and HG+DFX+FAC group. Light microscope was used to observe the morphology of the cells; cell proliferation was detected by Cell Counting Kit-8 assay, and Calcein-AM staining was used to detect the unstable iron pool (LIP) content; enzyme-linked immunosorbent assay reader was used to detect the reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and oxidized glutathione (GSSG); Western blot was used to detect the relative protein expression of Glutathione Peroxidase 4 (GPX4) and Solute Carrier Family 7 Member 11 (SLC7A11). Two-tailed Student t test was used for comparison between the two groups; one-way ANOVA was used for comparison between multiple groups. Results:Compared with the HG group and the HG+DFX+FAC group, the cell proliferation rate and the contents of GSH and the relative protein expression of GPX4, and SLC7A11 in the HG+DFX group were significantly increased, and the differences were statistically significant ( F=150.70, 21.02, 26.09, 52.62; P<0.001). The contents of LIP, ROS, MDA, and GSSG were significantly decreased, and the differences were statistically significant ( F=807.20, 16.94, 31.62, 19.21; P<0.001). Conclusions:High glucose significantly induces an increase in LIP, lipid peroxidation, and ferroptosis in HREC. Deferasirox inhibits lipid peroxidation and ferroptosis in HREC by downregulating LIP levels.
