Protective effects of escin and dextromethorphan on Alzheimer disease in Caenorhab-ditis elegans models
10.19723/j.issn.1671-167X.2025.04.022
- VernacularTitle:基于秀丽线虫模型探究七叶皂苷和右美沙芬对阿尔茨海默病的保护作用
- Author:
Yiping ZHANG
1
;
Ludi LI
;
An ZHU
;
Wusheng XIAO
;
Qi WANG
Author Information
1. 北京大学公共卫生学院毒理学系,国家中医药管理局中药配伍减毒重点研究室,食品安全毒理学研究与评价北京市重点实验室,北京 100191
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Escin;
Dextromethorphan;
Caenorhabditis elegans
- From:
Journal of Peking University(Health Sciences)
2025;57(4):764-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether escin(ESC)and dextromethorphan(DEX)have the protective effects on the progression and symptoms of Alzheimer disease(AD).Methods:The AD model of Caenorhabditis elegans(C.elegans)was established by transgenic amyloid β-protein(Aβ protein).Different concentrations of ESC or DEX or 50 μmol/L memantine(MEM)were used to treat the AD model worms,and their lifespan was detected.The movement ability of AD model C.elegans was evalua-ted by body bending frequency and head swinging frequency.The changes in cognitive functions of AD model C.elegans before and after treatment were detected by chemotaxis experiments.The changes in A βprotein and reactive oxygen species(ROS)content in C.elegans were detected.The changes in gene pathways related to oxidative stress were detected by Real-time quantitative polymerase chain reaction(RT-qPCR).Results:At high dose 1 000 μmol/L,ESC or DEX treatment showed no significant effects on the activity of C.elegans.Compared with untreated worms,the survival time of AD model C.elegans in the 20 μmol/L ESC and 60 μmol/L DEX intervention groups was significantly extended.In the middle stage of AD progression,the body bending frequency and head swinging frequency of AD model worms after ESC or DEX treatment was significantly increased compared with the untreated control group with DEX being more effective in the recovery of head swinging frequency.For the early cognitive function tests,the chemotaxis index of ESC or DEX treated worms was significantly higher than that of the untreated worms,which correlated with marked reductions in the Aβ protein levels.The reactive oxygen species content in the drug intervention group was also lower than that in the control group.RT-qPCR results showed that ESC could inhibit oxidative stress in the AD model C.elegans by a 2-fold upregulation of skn1 expression.Conclusion:ESC and DEX could improve the reductions of movement ability and cognitive function in the AD model worms and delay the aggravation of AD-related symptoms.ESC delays the progression of AD pos-sibly by activating the SKN-1/Nrf2 pathway to protect against oxidative injury in the AD model.
