Changpu Yujin Tang alleviates neuroinflammation in rats with Tourette syndrome by inhibiting the NLRP3/Caspase-1/GSDMD signaling pathway
10.13431/j.cnki.immunol.j.20250034
- VernacularTitle:菖蒲郁金汤通过抑制NLRP3/Caspase-1/GSDMD信号通路减轻多发性抽动症大鼠的神经炎症
- Author:
Shuang HUANG
1
;
Mengxue LI
;
Liwei HUANG
;
Mingyang SUN
;
Kexin SUN
;
Xing WEI
;
Xiao LIU
;
Huan LYU
;
Zhenggang SHI
Author Information
1. 730000 兰州,甘肃中医药大学中医临床学院;400051,重庆市九龙坡区中医院儿科
- Publication Type:Journal Article
- Keywords:
Tourette syndrome;
Changpu Yujin Tang;
NLRP3/Caspase-1/GSDMD signaling pathway;
Neuroinflammation
- From:
Immunological Journal
2025;41(4):231-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and mechanisms of Changpu Yujin Tang(CPYJT)on the NOD-like receptor thermal protein domain associated protein 3/cysteinyl aspartate specific proteinase-1/Gasdermin D(NLRP3/Caspase-1/GSDMD)signaling pathway-mediated neuroinflammation in rats with Tourette syndrome(TS).Methods SPF-grade male SD rats were randomly divided into the Control and TS groups.After successful modeling in the TS group,the rats were randomly divided into the Model,Tiapride,and CPYJT groups,and were treated with the corresponding drugs for 4 weeks.After the treatment,the rats' behavior was scored,H & E staining was used to observe pathological changes in the striatum,ELISA was used to measure the content of IL-1β and IL-18,RT-qPCR was used to detect the expression of NLRP3 and ASC mRNA,and Western blot was used to detect the expression of NLRP3,ASC,Caspase-1,Cleaved-Caspase-1,GSDMD,and GSDMD-NT proteins.Results Compared with the Control group,the Model group showed increased scores of stereotyped and motor behaviors(P＜0.01),pathological changes in the striatal tissue,increased content of IL-1β and IL-18(P＜0.01),and upregulated expression of NLRP3,ASC mRNA,and NLRP3,ASC,Caspase-1,Cleaved-Caspase-1,GSDMD,and GSDMD-NT proteins(P＜0.01).Compared with the Model group,the Tiapride group and the CPYJT group showed decreased scores of stereotyped and motor behaviors(P＜0.01),improved pathological damage in the striatal tissue,reduced content of IL-1β and IL-18(P＜0.01),and inhibited expression of NLRP3,ASC mRNA,and NLRP3,ASC,Caspase-1,Cleaved-Caspase-1,GSDMD,and GSDMD-NT proteins(P＜0.01).Conclusion The therapeutic effect of CPYJT on TS is related to the inhibition of the neuroinflammatory response mediated by the NLRP3/Caspase-1/GSDMD signaling pathway.
