Therapeutic effects of picroside Ⅱ on diabetes nephropathy rats by regulating fatty acid synthase(Fas)/fatty acid synthase ligand(FasL)signaling pathway
10.13431/j.cnki.immunol.j.20250032
- VernacularTitle:胡黄连苷Ⅱ调节脂肪酸合成酶/脂肪酸合成酶配体信号通路对糖尿病肾病大鼠的治疗作用
- Author:
Zhao DONG
1
;
Jian HOU
;
Shifang WU
;
Yan LIU
;
Zhihu GUO
;
Xuenan LIU
;
Chao ZHENG
;
Weihuan ZHANG
Author Information
1. 063000 唐山,开滦总医院内分泌二科
- Publication Type:Journal Article
- Keywords:
Picroside Ⅱ;
Fatty acid synthase/fatty acid synthase ligand signaling pathway;
Diabetes nephropathy;
Therapeutic effects
- From:
Immunological Journal
2025;41(4):217-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the therapeutic effect of picroside Ⅱ(P Ⅱ)on diabetes nephropathy(DN)rats by regulating fatty acid synthase(Fas)/fatty acid synthase ligand(FasL)signaling pathway.Methods A DN rat model was constructed by combining high sugar and high-fat diet with streptozotocin(STZ)injection.DN rats were grouped into model group(DN group),low,medium and high dose picroside Ⅱ groups(P-L group,P-M group,P-H group),and high dose picroside Ⅱ+Fas recombinant protein group(P-H+rh-Fas group),with healthy rats as control group,and 18 rats in each group.Fasting blood glucose(FBG),body mass,24-hour urinary protein(24h UTP),and renal function(SCr,BUN)were measured in rats.Hematoxylin-eosin(HE)staining and Masson staining were applied to observe the pathological changes in renal tissue of rats;immunohistochemistry and Western blot were applied to detect the expression of RAGE,COL-Ⅳ and pathway proteins,respectively.Results Compared with the control group,rats in DN group showed thickening of the glomerular basement membrane,mesangial proliferation,tubular degeneration,dilation,atrophy,fatty degeneration,obvious collagen deposition,higher levels of FBG,24h UTP,SCr,BUN,TNF-α,IL-6 and IL-1β,body mass loss,and higher expression of RAGE,COL-Ⅳ,Fas and FasL(P＜0.05).Compared with the DN group,the glomerular and tubular lesions were reduced and collagen deposition was decreased in the P-L,P-M and P-H groups,furthermore,the FBG,24h UTP,SCr,BUN,TNF-α,IL-6,IL-1β levels were lower,body mass was higher,and the RAGE,COL-Ⅳ,Fas,FasL expression was lower(P＜0.05).Compared with the P-H group,the renal tissue lesions in the P-H+rh-Fas group worsened,the FBG,24 h UTP,SCr,BUN,TNF-α,IL-6,IL-1β levels were higher,body mass was lower,and the RAGE,COL-Ⅳ,Fas,FasL expression was higher(P＜0.05).Conclusion Picroside Ⅱ exerts therapeutic effects on DN rats by inhibiting the Fas/FasL signaling pathway.
