Huanglian Ganjiang Decoction regulates mitochondrial apoptosis through regulation of NF-κB/CXCL1/CXCR2 pathway to improve symptoms in ulcerative colitis mice
10.13431/j.cnki.immunol.j.20250031
- VernacularTitle:黄连干姜汤调控NF-κB/CXCL1/CXCR2通路和线粒体凋亡改善小鼠溃疡性结肠炎症状
- Author:
Wenli WEI
1
;
Shijie REN
;
Xingchi LIU
;
Jingyuan LIU
;
Sujie JIA
;
Xiaomeng LANG
;
Heying WANG
;
Jianping LIU
Author Information
1. 050091 石家庄,河北中医药大学研究生院;050011 石家庄,河北省中西医结合胃肠病研究重点实验室
- Publication Type:Journal Article
- Keywords:
Huanglian Ganjiang Decoction;
Ulcerative colitis;
Mitochondrial apoptosis;
NF-κB/CXCL1/CXCR2 pathway
- From:
Immunological Journal
2025;41(4):209-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Huanglian Ganjiang Decoction in regulating the mitochondrial apoptotic pathway through the NF-κB/CXCL1/CXCR2 pathway in ulcerative colitis(UC).Methods Sixty C57BL/6J mice were divided into normal group,model group,Huanglian Ganzhang Decoction groups(low,medium and high doses)and mesalazine group.Except for the normal group,UC models were established in the other groups.The general conditions of the mice of all groups were recorded.HE staining was used to observe the pathological changes of the colon and rectum;electron microscopy was used to observe the mitochondrial condition;ELISA was used to determine the contents of IL-6,IL-10,D-lactic acid and DAO;Real-time PCR was used to detect the mRNA expression of NF-κB p65,CXCL1,CXCR2,Bcl-2 and Bax;Western blot was used to determine the protein expression levels of NF-κB p65,CXCL1,CXCR2,p-JAK2,p-STAT3,Bcl-2 and Bax.Results Compared with the normal group,the model group of mice showed a decline in condition,shortened length of the colon and rectum,and mitochondrial structural damage,furthermore,the levels of IL-6,DAO and D-lactic acid were increased,IL-10 level was decreased,the mRNA expression of NF-κB p65,CXCL1,CXCR2 and Bax were enhanced,Bcl-2 mRNA expression was weakened,the protein expression of NF-κB p65,CXCL1,CXCR2,p-JAK2,p-STAT3 and Bax were enhanced,Bcl-2 protein expression was weakened.Compared with the model group,the indexes mentioned above were reversed in the drug groups,especially in the high-dose group.Conclusion Huanglian Ganjiang Decoction may exert its therapeutic effect on UC in mice by regulating the NF-κB/CXCL1/CXCR2 pathway and mitochondrial apoptotic pathway.
