miR-129-1-3p inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells via BMP2/SMAD1 signaling pathway
10.13591/j.cnki.kqyx.2025.06.004
- VernacularTitle:miR-129-1-3p通过BMP2/SMAD1信号通路抑制人骨髓间充质干细胞成骨分化的研究
- Author:
Mingzhu GENG
1
;
Wenqing MU
1
;
Lin QIU
1
;
Wei ZHANG
1
Author Information
1. 南京医科大学附属口腔医院口腔特诊科,江苏南京(210029);口腔疾病研究与防治国家级重点实验室培育建设点,江苏南京(210029);江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Publication Type:Journal Article
- Keywords:
miR-129-1-3p;
BMP2/SMAD1 signaling pathway;
human bone marrow mesenchymal stem cells;
osteogenic differentia-tion;
osteoporosis
- From:
STOMATOLOGY
2025;45(6):418-423,429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-129-1-3p on the osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs)and its potential mechanism.Methods Negative control group,the miR-129-1-3p mimic group,the miR-129-1-3p inhibitor group and the corresponding negative control were constructed and transfected into hBMSCs.The formation of calcium-mineralized nodules was observed by alkaline phosphatase staining and alizarin red S staining.The expression levels of miR-129-1-3p and osteogenic differentiation markers were detected by qRT-PCR.Western blot detected the protein expressions of bone mor-phogenetic protein 2(BMP2),SMAD1 and p-SMAD1.Results After transfection,the expression level of miR-129-1-3p in mimic group was significantly increased(P＜0.05),the number of mineralized nodules was significantly decreased,and the expression levels ofBMP2,Runt-related transcription factor 2(RUNX2),osteocalcin(OCN)mRNA were significantly down-regulated(P＜0.05).BMP2 and p-SMAD1 protein were also significantly down-regulated(P＜0.05)compared with Mimic-NC group.The expression levels of BMP2,RUNX2,OCN mRNA were significantly up-regulated in inhibitor group(P＜0.05)compared with Inhibitor-NC group.BMP2 and p-SMAD1 protein were significantly up-regulated in inhibitor group(P＜0.05).Conclusion miR-129-1-3p can inhibit the osteo-genic differentiation of human bone marrow mesenchymal stem cells by suppressing BMP2/SMAD1 signaling pathway.
