Identification of in vivo Components and Distribution Characteristics in Rats,Cardiac Tissues of Zhigancao Decoction by UP-LC-Q-TOF/MS
10.14148/j.issn.1672-0482.2025.0896
- VernacularTitle:基于UPLC-Q-TOF/MS分析炙甘草汤体内成分及心脏组织分布特征
- Author:
Zhiling TANG
1
;
Yao LI
;
Hao ZHI
;
Jingyan WANG
;
Xueting CAI
;
Peng CAO
;
Qian ZHOU
Author Information
1. 南京中医药大学附属中西医结合医院,江苏 南京 210028;南京中医药大学药学院,江苏 南京 210023
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF/MS;
Zhigancao Decoction;
blood-absorbed components;
metabolites;
heart
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(7):896-903
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To deeply explore the in vivo pharmacodynamic substance basis of Zhigancao Decoction,a classic tradi-tional Chinese medicine formula,and provide scientific evidence for its rational application and development in modern clinical practice.METHODS Wistar rats were treated with 12.15 g·kg-1 Zhigancao Decoction by gavage.Rat plasma samples were collect-ed at 10 time points(5,15,30,60,120,240,360,480,600 and 720 min after administration)and rat heart(atrial and ventricu-lar)tissue samples were collected at 12 h after administration.Components in the plasma and heart samples were qualitatively identi-fied by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS),and the distri-bution characteristics of Zhigancao Decoction in vivo were analyzed.At the same time,the time-concentration curve of the prototype components and metabolites in Zhigancao Decoction was drawn to observe the changes of blood drug concentration.RESULTS A total of 11 prototype components(Ajugol,Nicotiflorin,Isoschaftoside,4-Hydroxycinnamic acid,Rehmapicrogenin,4-Hydroxybenzoic acid,4′,7-Dihydroxyflavone,Calycosin,3′,4′,7-Trihydroxyflavone,Pinellic acid,Truxillic acid)and 7 metabolites were identified from the plasma samples of Zhigancao Decoction,mainly including flavonoids(flavonoids glycosides),organic acids,and iridoid glyco-sides,etc.Additionally,6 prototype components(Ajugol,Isoschaftoside,Rehmapicrogenin,4′,7-Dihydroxyflavone,Liquiritigenin,3′,4′,7-Trihydroxyflavone)and 3 metabolites were identified from the cardiac samples(the atrium and the ventricle showed the same results).The metabolic pathways mainly involved Phase Ⅰ metabolism and glucuronidation.CONCLUSION The prototype compo-nents and metabolites in plasma and heart tissue of Zhigancao Decoction is preliminarily determined,providing a reference for analyzing the active components of Zhigancao Decoction in heart tissue.
