Crocetin inhibits diabetic retinopathy by down-regulating the activity of STAT3
10.13389/j.cnki.rao.2025.0092
- VernacularTitle:藏红花酸通过下调STAT3活性抑制糖尿病视网膜病变
- Author:
Lina YANG
1
;
Hui KONG
;
Ping JIA
Author Information
1. 264006 山东省烟台市,烟台业达医院/烟台眼科医院
- Publication Type:Journal Article
- Keywords:
crocetin;
diabetic retinopathy;
retinal pigment epithelial cells;
signal transducer and activator of tran-scription 3;
oxidative stress;
inflammation
- From:
Recent Advances in Ophthalmology
2025;45(7):526-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential value of crocetin(CRO)in the treatment of diabetic retinopathy(DR)and its effect on signal transducer and activator of transcription 3(STAT3)activity.Methods(1)In vitro experi-ment:RPE cells were divided into C group,HG group,HG+5/10/20CRO groups and HG+20CRO+Colivelin TFA(C-TFA)group.Cells in the C group were cultured with normal glucose medium(5 mmol·L-1).Cells in the HG+5/10/20CRO groups were cultured in high glucose medium(25 mmol·L-1)with 5,10 and 20 μmol·L-1 CRO added.Cells in the HG+20CRO+C-TFA group were cultured in high glucose medium with 20 μmol·L-1 CRO and 0.5 μmol·L-1 C-TFA added.The cells in each group were cultured for 48 h.Cell viability,proliferation and apoptosis were detected using MTT,EdU staining,and TUNEL,respectively.Meanwhile,the levels of oxidative stress and inflammatory factors were detected by ELISA,and the activation level of STAT3 was detected by Western blot.(2)In vivo experiment:Rats were divided into 6 groups:NC group,DR group,20/40/80CRO groups and 80CRO+C-TFA group.NC group was normal control rats,and the other groups were STZ-induced DR model rats.NC group and DR group were gavaged with 5 g·L-1 CMC-Na.20/40/80CRO groups were gavaged with 20,40 and 80 mg·kg-1 of CRO.80CRO+C-TFA group was gavaged with 80 mg·kg-1 of CRO,and 1 mg·kg-1 of C-TFA was injected intraperitoneally.The treatment cycle was 4 weeks.HE staining and TUNEL staining were employed to examine retinal morphology and apoptosis.The concentrations of oxidative stress markers and inflammatory factors in the retina were measured using ELISA,while the activation level of STAT3 in the retina was as-sessed through Western blot analysis.Results(1)In vitro experiments:Compared with the HG group,the HG+5/10/20CRO groups exhibited increased relative cell viability,SOD and GPx activities,and EdU+cell ratio,but decreased TUNEL+cell ratio,MDA content,TNF-α,IL-1β and IL-6 levels,and STAT3 phosphorylation level(P＜0.05).Compared with the HG+20CRO group,the abovementioned indicators of RPE cells in the HG+20CRO+C-TFA group were reversed(all P＜0.05).(2)In vivo experiments:Compared with the DR group,the 20/40/80CRO groups exhibited increased SOD and GPx activities,but decreased proportion of retinal TUNEL+cells,retinal MDA content,TNF-α,IL-1β and IL-6 levels,and retinal STAT3 phosphorylation level(all P＜0.05).Compared with the 80CRO group,the abovementioned indicators of reti-nal cell in the 80CRO+C-TFA group were reversed(all P＜0.05).Conclusion CRO can alleviate high glucose-induced rat RPE cell injury and retinal injury of DR rats by inhibiting STAT3 activity.
