Analysis of the spectrum-efficacy correlation and pharmacodynamic material basis of green tea extract intervention in experimental age-related macular degeneration based on UPLC-Q-Exactive Orbitrap MS
10.13389/j.cnki.rao.2025.0091
- VernacularTitle:基于UPLC-Q-Exactive Orbitrap MS的绿茶提取物干预湿性年龄相关性黄斑变性的谱-效相关药效物质基础解析
- Author:
Yifei WANG
1
;
Haiyang XU
1
;
Yan GAO
1
;
Bonian ZHAO
1
Author Information
1. 250355 山东省济南市,山东中医药大学药物研究院,国家中医药管理局高水平重点学科-中药药剂学,黄河流域特色中药生态保护和高质量发展协同创新中心
- Publication Type:Journal Article
- Keywords:
green tea;
UPLC-Q-Exactive Orbitrap MS;
age-related macular degeneration;
spectrum-effect relation-ship;
pharmacological substance basis
- From:
Recent Advances in Ophthalmology
2025;45(7):518-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the spectrum-efficacy relationship between the fingerprint of green tea extract and its pharmacological effects in intervention of neovascular age-related macular degeneration(nAMD),explore the phar-macodynamic material basis,and identify key active components.Methods The fingerprint of green tea extract was es-tablished using UPLC-Q-Exactive Orbitrap MS.Hierarchical cluster analysis and orthogonal partial least squares discrimi-nant analysis were used to explore differences in chemical composition among green tea samples from different origins.A zebrafish nAMD model induced by cobalt chloride was established to evaluate the pharmacological activity of green tea ex-tract in nAMD intervention.Partial least squares regression analysis(PLSR)and grey relational analysis(GRA)were em-ployed to study the spectrum-efficacy correlation,comprehensively analyze the pharmacodynamic material basis of green tea in AMD intervention,and screen for key active components.Results A fingerprint detection method for green tea ex-tract was successfully established,and 31 chemical components were qualitatively and quantitatively characterized.Hierar-chical cluster analysis and orthogonal partial least squares discriminant analysis classified 50 batches of green tea samples into two major categories,identifying 15 key differential components.Pharmacological experiments demonstrated that green tea extract inhibited abnormal vascular growth in the zebrafish retina caused by cobalt chloride.Through PLSR and GRA,six components of green tea extract were found to be significantly correlated with pharmacological activity and showed high variable importance in projection:quinic acid,epicatechin,gallocatechin,epigallocatechin,gallocatechin gal-late,and epigallocatechin gallate.Conclusion This study comprehensively characterized 31 chemical components in green tea.Based on the nAMD zebrafish model and spectrum-effect correlation theory,PLSR and GRA analyses were ap-plied to accurately identify quinic acid,epicatechin,gallocatechin,epigallocatechin,gallocatechin gallate,and epigallocate-chin gallate as the key effective substances in green tea for intervening in nAMD.This provides a foundation for the devel-opment of new drugs with therapeutic potential against nAMD,and offers data support for establishing a comprehensive quality evaluation system for green tea.
