Liver organoid technology and its related progress in liver disease research
10.3760/cma.j.cn501113-20241111-00572
- VernacularTitle:肝脏类器官技术及其在肝病研究中的相关进展
- Author:
Zerui WANG
1
;
Yingying YU
;
Weiguo HONG
;
Fusheng WANG
;
Enqiang LINGHU
Author Information
1. 解放军总医院第一医学中心消化内科医学部，北京 100853
- Publication Type:Journal Article
- Keywords:
Pluripotent stem cell;
Liver transplantation;
Liver cancer;
Precision medicine;
Regeneration medicine;
Liver organoids;
Drug screening
- From:
Chinese Journal of Hepatology
2025;33(2):108-114
- CountryChina
- Language:Chinese
-
Abstract:
Liver transplantation, hepatocyte transplantation, and bioartificial liver are means of treating end-stage liver disease and acute liver failure. However, insufficient liver resources and immune rejection after transplantation, as well as a shortage of liver tissue or cell donors, are challenges faced in clinical treatment. The research progress of liver organoid technology in recent years may provide new ways to solve the above problems. Organoids are a kind of three-dimensional cell condensates formed by the self-organization of pluripotent or adult stem cells through three-dimensional culture in vitro, which can imitate the spatial structure and physiological function characteristics of the original organs, can be sub-cultured in vitro, replicated on a large scale, and have the ability of self-renewal. The emergence of organoid technology has brought new hope for providing mechanism exploration models and resolution of hepatocyte resources. In particular, induced pluripotent stem cell-derived organoids do not involve ethical concerns, and are combined with emerging technologies such as gene editing and organ chips, thereby overcoming the constraint of traditional disease research models and establishing a new platform for translational medicine. Additionally, it has expansive application prospects in building disease models, screening drugs, precision medicine, regenerative medicine, and organ transplantation. Thus, this paper summarizes the technical principles, preclinical research, and application progress and challenges of liver organoid technology.
