Influence of influenza A virus on macrophages based on JAK1/2-STAT1 signaling pathway and intervention effects of Ma Xing Shigan decoction drug containing serum
10.3969/j.issn.1000-484X.2025.07.027
- VernacularTitle:从JAK1/2-STAT1信号通路探讨A型流感病毒对巨噬细胞的影响及麻杏石甘汤含药血清的干预作用
- Author:
Chunjing CHEN
1
;
Cheng ZHAO
;
Fangguo LU
;
Xianggang ZHANG
;
Li HE
;
Meihong PENG
;
Bei CHEN
;
Tong LI
Author Information
1. 湖南中医药大学医学院,长沙 410208
- Publication Type:Journal Article
- Keywords:
RAW264.7;
IAV;
Ma Xing Shigan decoction;
JAK1/2-STAT1;
Inflammatory factors;
Immunopathological injury
- From:
Chinese Journal of Immunology
2025;41(7):1730-1736,1751
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of influenza A virus(IAV)on macrophages based on expression of cytokines mediated by Janus kinase/signal transducer and activator of transcription(JAK/STAT)pathway,and to further explore the intervention effects of Ma Xing Shigan decoction drug containing serum.Methods:RAW264.7 was divided into control group,JAK/STAT signal pathway activator group,inhibitor group,model group,oseltamivir group,antiviral granule group and Ma Xing Shigan decoction group.Cell samples were collected after the intervention of IAV and subsequent treating of drug-containing serum for 24 and 48 hours.Secretion levels of IL-1β and CXCL2 in supernatant were detected by ELISA.mRNA expression levels of influenza virus Nuclear Pro-tein(NP),JAK1/2 and STAT1 of cells were detected by RT-qPCR.Protein expression levels of JAK1/2 and STAT1 were detected by Western blot,and protein expression levels of p-STAT1 was detected by immunofluorescence.Correlation between protein expression levels of p-STAT1 and secretion of IL-1β and CXCL2 were evaluated by Pearson correlation analysis.Results:Secretion levels of IL-1β and CXCL2,mRNA expression levels of NP,JAK1/2,STAT1,protein levels of JAK1/2,STAT1 and p-STAT1 were increased,and protein levels of p-STAT1 was positively correlated with the secretion of IL-1β and CXCL2.Ma Xing Shigan decoction could down-regulate secretions of IL-1β and CXCL2,mRNA expression levels of NP,JAK1/2 and STAT1,protein expression levels of JAK1,JAK2,STAT1 and p-STAT1.Conclusion:Activation of JAK1/2-STAT1 signal pathway and imbalance of inflammatory factors may be one of the molecular mechanisms of immunopathological damage of macrophages induced by IAV.Ma Xing Shigan decoction may alle-viate pathogenic effects of IAV by regulating this signal pathway.
