Effects of Shaoyao Gancao Decoction on autophagy following post-ERCP pancreatitis in rats
10.3969/j.issn.1001-1528.2025.03.009
- VernacularTitle:芍药甘草汤对ERCP术后胰腺炎大鼠胰腺自噬水平的影响
- Author:
Meng CHEN
1
;
Gan CAI
1
;
Biao GONG
1
;
Xi-wen ZHANG
1
;
Chan LÜ
1
;
Tao LI
1
;
Yong-hong HU
1
;
Fu LI
1
Author Information
1. 上海中医药大学附属曙光医院,上海 201203
- Publication Type:Journal Article
- Keywords:
Shaoyao Gancao Decoction;
post-ERCP pancreatitis;
autophagy
- From:
Chinese Traditional Patent Medicine
2025;47(3):759-766
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the mechanism of Shaoyao Gancao Decoction in preventing meglumine diatrizoate-induced post-ERCP pancreatitis in rats through autophagy regulation.METHODS The rats were randomized into the normal group,the model group,the low-dose and high-dose Shaoyao Gancao Decoction(1.5,3.0 g/kg),and the indomethacin suppository group.A rat model of post-ERCP pancreatitis was induced by meglumine diatrizoate injection into the pancreatic duct under continuous pressure.The rats had their pancreatic tissues stained with HE to observe the pathological alterations,inflammatory cell infiltration,hemorrhage and necrosis;their serum levels of IL-1β,IL-6,IL-8,TNF-α,AMS,and IL-10 identified by ELISA;their autophagic vacuoles in pancreatic acinar cells observed by transmission electron microscopy;their pancreatic protein expressions of Beclin1,LC3B,p62,TRAF2 and p-JNK detected by IHC and Western blot;and their pancreatic mRNA expressions of Beclin1 and TRAF2 detected by RT-qPCR.RESULTS Compared with the model group,the high-dose Shaoyao Gancao Decoction group displayed no obvious hemorrhage;improvement in edema of acinar and interstitial cells;obviously less cellular inflammatory infiltration;substantially decreased serum levels of IL-1β,IL-6,TNF-α and AMS(P＜0.05,P＜0.01);drastically reduced amount of autophagosomes in acinar cells;and down-regulated expressions of autophagy-related proteins Beclin1,LC3,p62,TRAF2 and p-JNK(P＜0.05,P＜0.01).CONCLUSION Shaoyao Gancao Decoction can prevent post-ERCP pancreatitis by ameliorating pancreatic tissue injury,decreasing serum inflammatory response level,and interfering with abnormal autophagy of pancreatic acinar cells.Its molecular mechanism may involve inhibition of TRAF2 protein expression and modulation of p-JNK activation.
