Macrophage subtype in mouse photoaged skin: dynamics and regulatory pathways
10.3760/cma.j.cn114657-20241113-00186
- VernacularTitle:巨噬细胞亚型在小鼠光老化皮肤中的改变和调控机制分析
- Author:
Zuochao YAO
1
;
Lu LU
1
;
Jianghui YING
1
;
Hua JIANG
1
;
Hui WANG
1
Author Information
1. 同济大学附属东方医院整形外科，上海 200120
- Publication Type:Journal Article
- Keywords:
Photoaging;
Ultraviolet ray;
Inflammation;
Macrophage;
Polarization
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2025;31(6):611-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the alteration and regulatory of macrophage subtypes and the underlying mechanisms of cellular interactions in mouse photoaged skin.Methods:Immune cell type identification was performed by estimating relative subpopulations of RNA transcripts (CIBERSORT) on 18 samples from the public dataset GSE58915. A total of 15 healthy male C57BL/6J mice aged 6-8 weeks were exposed to an animal UV-radiation chamber for 4 weeks (4W-UV group) and 8 weeks (8W-UV group). Skin samples were collected for hematoxylin-eosin staining, Masson staining, immunohistochemistry and immunofluorescence to evaluate skin architecture, inflammatory status and macrophage infiltration. Dermal fibroblasts of passages 3-5 were irradiated daily at 36 mW/cm2 for 7 days to establish a photoaged model; senescence-associated indicators were detected by β-galactosidase staining and Western blot. A co-culture system of photoaged fibroblasts and mouse monocyte-macrophages was then constructed; phagocytosis assays and flow cytometry were employed to determine the phagocytic capacity and polarization of monocyte-macrophages.Results:The number of M1 macrophages in mouse skin increased with UV-radiation duration; M1 counts in the 8W-UV and 4W-UV groups were (17.2±4.7) and (10.3±2.1) cells/HPF, respectively, both higher than the (3.8±0.7) cells/HPF observed in the control group (both P<0.01). Monocyte-macrophages treated with supernatant from photoaged fibroblasts exhibited enhanced phagocytic activity and a higher proportion of CD86-positive cells. Conclusions:Prolonged UV radiation aggravates photoaging and increases M1-macrophage infiltration in skin tissue. Cytokines secreted by photoaged fibroblasts induce M1 polarization of macrophages.
