Analysis of dynamic change patterns of six mycotoxin contents during the fermentation of Massa Medicata Fermentata
10.3969/j.issn.1001-1528.2025.03.006
- VernacularTitle:六神曲在发酵过程中6种真菌毒素含量的动态变化规律分析
- Author:
Shuang WANG
1
;
Li ZHOU
;
Hai-yan SHI
;
Xia ZHAO
;
Yan-wei CUI
;
Hua-yin BAO
;
Nan XU
Author Information
1. 山东中医药大学药学院,山东济南 250355;山东省中医药研究院中药制剂研究所,山东济南 250014
- Publication Type:Journal Article
- Keywords:
Massa Medicata Fermentata;
fermentation;
mycotoxins;
content determination;
dynamic change patterns;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(3):740-744
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze the dynamic change patterns of aflatoxin B1,aflatoxin B2,aflatoxin G1,aflatoxin G2,T-2 toxin and deoxynivalenol contents during the fermentation of Massa Medicata Fermentata.METHODS The analysis was performed on a 40 ℃ thermostatic Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm),with the mobile phase comprising of 0.01％formic acid-[acetonitrile-methanol(1∶1)]flowing at 0.3 mL/min,and electron spray ionization source was adopted in positive ion scanning with multiple reaction monitoring mode.RESULTS Six mycotoxins showed good linear relationships within their own ranges(R2＞0.998 0),whose average recoveries were 76.1％-119.3％with the RSDs of 0.49％-9.27％,and except for deoxynivalenol,their contents demonstrated the trends of growing out of nothing and gradually increasing.CONCLUSION The risk of mycotoxin infection exists in the fermentation of Massa Medicata Fermentata.This simple,efficient,rapid and sensitive method can provide a reference for whole-process monitoring the fermentation process for Massa Medicata Fermentata.
