Expressions of miR-29b and Klotho in peripheral blood of chronic obstructive pulmonary disease patients complicated with pulmonary infection and their clinical significance
10.11816/cn.ni.2025-241052
- VernacularTitle:慢性阻塞性肺疾病合并肺部感染外周血miR-29b和Klotho表达及意义
- Author:
Zhenzhen GUI
1
;
Jie QIU
1
;
Jie LIU
1
;
Xia CAO
1
Author Information
1. 宁夏医科大学总医院呼吸与危重症医学科,宁夏银川 750004
- Publication Type:Journal Article
- Keywords:
Chronic obstructive pulmonary disease;
Pulmonary infection;
Microribonucleic acid-29b;
Klotho;
Pathogen
- From:
Chinese Journal of Nosocomiology
2025;35(6):840-844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the expression levels of microribonucleic acid(miR)-29b and soluble Klotho protein in peripheral blood of the chronic obstructive pulmonary disease(COPD)patients complicated with pulmo-nary infection and analyze the clinical significance.METHODS The clinical data were retrospectively collected from 139 patients with COPD who were treated in Ningxia Medical University General Hospital from Feb.2022 to Feb.2024.The patients were divided into the infection group with 41 cases and the no infection group with 98 cases ac-cording to the status of pulmonary infection.The distribution of pathogens isolated from the patients of the infec-tion group was observed.The levels of serum miR-29b and Klotho were compared between the infection group and the no infection group or of the infection group before and after the anti-infection treatment.The efficiencies of ser-um miR-29b and Klotho in prediction of pulmonary infection were analyzed by means of receiver operating charac-teristic(ROC)curves.RESULTS Totally 46 strains of pathogens were isolated from the infection group,67.39％of which were gram-negative bacteria,26.09％were gram-positive bacteria,and 6.52％were fungi.There were significant differences in the levels of serum miR-29b and Klotho between the infection group and the no infection group(P＜0.05);the expression level of serum miR-29b of the patients in the infection group who were effective for the anti-infection treatment was lower after the treatment than before the treatment,while the expression level of Klotho was higher after the treatment than before the treatment(P＜0.05);the expression level of miR-29b of the patients effective for the treatment was lower than that of the patients ineffective for the treatment,while the expression level of Klotho of the patients effective for the treatment was higher than that of the patients ineffective for the treatment(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of the joint detec-tion of serum miR-29b and Klotho was 0.927 in prediction of pulmonary infection in the COPD patients,higher than 0.837 and 0.852 of the single detection.CONCLUSIONS The COPD patients complicated with pulmonary in-fection show the high expression level of serum miR-29b and low expression level of Klotho.The joint detection of the two indexes has high efficiency in prediction of pulmonary infection in the COPD patients,which can be used as reliable indexes for assessment of the curative effect after the anti-infection treatment.
